Comparative assessment of bioactive compounds, antioxidant capacity and nutritional quality of red seaweeds and water spinach
Introduction
In recent years, researchers are looking for cheaper sources of natural antioxidants and bioactive compounds. Several previous studies were accompanied to study the phytochemicals and antioxidant activity of natural sources like fruits, vegetables, plants, etc. Holdt and Kraan (2011) and Zzaman et al. (2021). However, constituents of phytochemicals in plants, especially in green leafy vegetables, raised interest among researchers, consumers, producers, and all relevant stakeholders. Among various Bangladeshi freshwater green leafy vegetables, water spinach is one of the most abundant but it is underused- people may not understand its value in terms of nutritional quality and antioxidant properties, which originates from China for the first time (Gupta et al., 2005). On the other hand, seaweeds have been used in Japan and China since the fourth and sixth centuries, respectively, as an essential diet. Besides, Asian people eat seaweed, and it is widespread in Korea (Gates, 2010). In contrast, Bangladeshi people have used seaweed slightly due to insufficient knowledge about its qualities. The nutritional value, taste consistency, colour and texture support seaweed as a healthy diet. Some edible seaweeds contain essential proteins, lipids, minerals, vitamins and possess rich antioxidant properties (Norziah and Ching, 2000). Seaweed nutrient content varies by species, geographic location, season, moisture, and temperature (Kaehler and Kennish, 1996). Seaweed has potential antibacterial, fungal, antioxidant and anti-viral activity (Venugopal, 2008). Compared with other marine resources, seaweed can play a critical role in many ways. Better attention should be given to marine algae since marine algae may contribute to future global food security for the ever-growing (Moomaw, 2017). Red seaweeds (under the Rhodophyta group) possess high protein levels, which was selected in this study (Cian et al., 2015). Consequently, this work focuses on assessing the nutritional properties of two seaweed species, i.e., Gelidium and Hypnea, accessible in almost every country on the coast. Bangladesh’s east coast, in particular, is filled with seaweed.
Water spinach is a major part of any healthy diet in green freshwater vegetables and is a good source of nutrients, particularly for more impoverished families due to low prices and abundance (Fu et al., 2011). Ipomoea is a freshwater weed typically found in wet monsoon (summer) to dry season (winter). It can also grow without any intensive care enriched with bioactive components (likely carotenoids, phenolic content, etc.) and act as a degenerative disease barrier (Gates, 2010). I. aquatica is a sizeable aquatic crop in Southern Asia, India, and southern China. It is an enduring watery herb in the Convolvulaceae group (Umar et al., 2007a). Its drifting character has a trailing vine throughout the aquatic environment that can have a maximum length of 3 cm and no need to be attached with soil. Also, leaves and stems can grow to 15 cm tall (Zhang et al., 2014). In this analysis, the proximate composition and antioxidant properties of Ipomoea were selected as the freshwater weed to be contrasted with the seaweeds. Green vegetables are the key source of nutrition in most developing countries, with food scarcity and famine. Despite the nutritional and medicinal value of these plants, very limited studies were conducted.
Free radicals play a major role in the human body, causing disease by increasing oxidation. Nevertheless, the use of oxygen in the human body is varied by the individual; it may cause the condition to become imbalanced and produces reactive oxygen species (ROS), which lead to carcinogenicity (Gunathilake and Ranaweera, 2016). Naturally generated ROS could be naturalized by the virgin antioxidant that came into our body through the ingestion of various green vegetables, seaweeds, fruits, etc. Bhattacharjee and Islam (2014). Synthetic antioxidants may inject the human body to treat many diseases, whereas natural antioxidants are often preferred (Franco et al., 2019, Preedy and Patel, 2020). Although all bioactive compounds can play a vital role against ROS, carotenoid has significant impacts; it may function to reduce cancer, cataracts, and other degenerative diseases (Perera and Yen, 2007).
Carotenoids are essentially plant pigments important for colouring in various fruits and vegetables, but only responsible for yellow, orange, and red. Besides this, carotenoids can also serve as a source of vitamin A found in fruits and vegetables. Moreover, carotene is also a form of carotenoid that can function against ROS; generally, several degenerative diseases (Perera and Yen, 2007). Carotenoid, however, has contributed to the antiphotoxidant process, namely this antioxidant called 1,1-diphenyl-2-picrylhydrazyl (DPPH). DPPH stops RBC (red blood cell) from being killed and fat oxidized in the body (World Health et al, 2011). Thus the free radical scavenging activity was assessed in samples to determine whether the sample has sufficient antioxidant potential or not (Ajila et al., 2007). Total antioxidants, however, can delay and inhibit rancidity, slow the growth of oxidative products, and increase shelf-life. Total antioxidant capacity (TAC) is developed in the field of chemistry but has been applied in the area of biology and medicine. So, here we introduced to calculate the amount of TAC in both samples, i.e., water spinach (Ipomoea) and seaweed (Hypnea, Gelidium), and clearly showed the difference between two vegetables of water bodies. Ferric acid Reducing Antioxidant Power assay (FRAP) is an essential tool to measure the antioxidant activity of several polyphenols, vitamin C, Vit-E, uric acid, etc. Several studies were conducted to assess the effects of flavonoids. They found astonishing benefits, especially in reducing inflammatory reaction on heart valves, thereby declining the risks of infective endocarditis and rheumatic fever, among the major CVS (cardiovascular system) threats in our society (Rees et al., 2018). Polyphenols, which are consumed mostly in the form of flavonoids, have certainly reduced the risk of developing prostate cancer, arguably the second most prevalent neoplasm present in the elderly male population (El-Din and El-Ahwany, 2016). Tannic acid, well-documented antimicrobial biomedicine, is consumed by a larger share of our community by drinking tea (Chung et al., 1998). However, freshwater weed and seaweed could be potential sources of this essential agent (Tannin) prescribed by this study. An exogenously synthesized water-soluble vitamin is also present in seaweed (Nielsen et al., 2021). Given that the human body cannot generate ascorbic acid (vitamin C) (Marcus, 2013), this plant source can greatly value our health. In Bangladesh, the consumption of seaweed in meals supplement is rare compared to those of freshwater species due to the assumption that marine species contain less nutrition and unsafe to consume. Owing to that, we aim to provide data on the nutritional values of seaweed and compared them to the commonly available freshwater weed. Two red seaweed species that are abundant, i.e., Hypnea musciformis and Gelidium pusillum, have the most significant value (nutritional value, medicinal value, industrial value) compared to other species chosen in this study. This research thus aims to analyse the bioactive compounds, antioxidant activity, and nutritional composition of seaweeds and freshwater weed; and figure out the best one among these three species.
Section snippets
Study area
The sampling site was selected as Kolatoli, Cox’s Bazar, which is an eastern coastal region of Bangladesh. Furthermore, Cox’s Bazar is connected with the Bay of Bengal by the south. The study area is full-filled with seaweeds—about 193 seaweed species are found in Bangladesh (Sarkar et al., 2016b). About 155 seaweed species has been recorded in the Cox’s Bazar where at 70% of are including Rhodophyta group (red species) (Sarkar et al., 2016b). However, this resource cannot be utilized properly
Results and discussion
One of the untapped resources of Bangladesh is seaweed whereas its abundance is plenty in the coastal region of Bangladesh. Among 193 seaweed species, 19 were dominant including red algae species, i.e., Hypnea and Gelidium (Sarkar et al., 2016a). In addition, water spinach is also the most abundant freshwater green vegetable in Bangladesh (Prasad et al., 2008) which was also considered for a comparative analysis in this research. Data obtained from this study has provided insight on both
Conclusion
In order to infer a deduction on which species is best in the criterion of nutritional and antioxidant properties among the seaweed and freshwater weed examined. Several aspects are needed to be taken into account. Firstly, the three antioxidant parameters, namely: total antioxidant capacity, ferric reducing activity, and free radical scavenging activity, show a similar pattern with Hypnea and Gelidium high in antioxidant activity and lowest in Ipomoea, suggesting that the red seaweeds have
CRediT authorship contribution statement
Md. Solaiman Hossain: Conceptualization, Investigation, Writing - original draft , Supervision. Saad Al-din Sifat: Methodology, Data curation, Writing - original draft. Mohammad Afzal Hossain: Writing - reviewing and editing, Supervision. Sazlina Salleh: Writing - reviewing and editing. Mofazzal Hossain: Writing - reviewing and editing. Sharmin Akter: Visualization, Investigation. M. Belal Hossain: Writing - reviewing and editing.
Declaration of Competing Interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Acknowledgements
We would like to express our gratitude to the SUST Research Centre, Shahjalal University of Science and Technology (SUST), Sylhet, Bangladesh for funding research (Grant number: PS/2019/1/25). We are also thankful to the Department of Food Engineering and Tea Technology, SUST for providing the laboratory facility. Research would not be possible without Falcon International Limited’s authority to authorize the taking of samples of seaweed.
References (81)
- et al.
Bioactive compounds and antioxidant potential of mango peel extract
Food Chem.
(2007) - et al.
Evaluation of antioxidant activities of principal carotenoids available in water spinach (Ipomoea aquatica)
J. Food Compos. Anal.
(2011) - et al.
Antioxidant properties of methanol extract and its solvent fractions obtained from selected Indian red seaweeds
Bioresour. Technol.
(2008) - et al.
Antioxidative properties of 34 green leafy vegetables
J. Funct. Foods
(2016) - et al.
Effect of different drying temperatures on the moisture and phytochemical constituents of edible Irish brown seaweed
LWT-Food Sci. Technol.
(2011) - et al.
Analysis of nutrient and antinutrient content of underutilized green leafy vegetables
LWT-Food Sci. Technol.
(2005) - et al.
Seaweed in food products: biochemical and nutritional aspects
Trends Food Sci. Technol.
(1993) Marcus, J.B. (ed.), Culinary Nutrition
(2013)- et al.
Nutritional composition of edible seaweed Gracilaria changgi
Food Chem.
(2000) - et al.
Dietary fiber, amino acid, fatty acid and tocopherol contents of the edible seaweeds Ulva lactuca and Durvillaea antarctica
Food Chem.
(2006)
Standardization and quantification of total tannins, condensed tannin and soluble phlorotannins extracted from thirty-two drifted coastal macroalgae using high performance liquid chromatography
Bioresource Technol. Rep.
Facts about the formation of new antioxidants in natural samples after subcritical water extraction
Food Res. Int.
Nutritional evaluation of some subtropical red and green seaweeds: Part I—proximate composition, amino acid profiles and some physico-chemical properties
Food Chem.
Aquaculture wastewater quality improvement by water spinach (Ipomoea aquatica Forsskal) floating bed and ecological benefit assessment in ecological agriculture district
Aquac. Eng.
Application of immersion pre-treatments and drying temperatures to improve the comprehensive quality of pineapple (Ananas comosus) slices
Heliyon
Indigenous leafy vegetables: a potential source of -carotene and ascorbic acid
Int. J. Veg. Sci.
Official Methods of Analysis of AOAC International. Volume I, Agricultural Chemicals, Contaminants, Drugs/Edited By William Horwitz
Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from Western Nepal
Plants
Biochemical composition of marine macroalgae from Gangetic Delta at the apex of Bay of Bengal
Afr. J. Basic Appl. Sci.
Evaluation of free radical scavenging activity and antioxidant potential of a few popular green leafy vegetables of Malaysia
Int. J. Food Prop.
Seaweed antioxidants as novel ingredients for better health and food quality: bangladesh prospective
Proc. Pak. Acad. Sci.
Antioxidant activities and phenolic contents of three red seaweeds (Division: Rhodophyta) harvested from the Gulf of Mannar of Peninsular India
J. Food Sci. Technol.
Antioxidant activities and polyphenolics of various solvent extracts of red seaweed, Gracilaria changii
J. Appl. Phycol.
RETRACTED: Biochemical and Nanotechnological Studies in Selected Seaweeds of Chennai Coast
J. Appl. Pharm. Sci.
Flavonoid content of several vegetables and their antioxidant activity
J. Sci. Food Agric.
Tannins and human health: a review
Crit. Rev. Food Sci. Nutr.
Proteins and carbohydrates from red seaweeds: evidence for beneficial effects on gut function and microbiota
Mar. Drugs
Antioxidant and antiproliferative activities of heterofucans from the seaweed Sargassum filipendula
Mar. Drugs
An assessment of the antioxidant and antimicrobial activity of six species of edible Irish seaweeds
Int. Food Res. J.
Bioactivity and phytochemical constituents of marine red seaweeds (Jania rubens, Corallina mediterranea and Pterocladia capillacea)
J. Taibah Univ. Sci.
Penurunan logam berat dan pigmen pada pengolahan geluring rumput laut Gelidium sp. dan Ulva lactuca
J. Pengolahan Hasil Perikanan Indonesia
Antioxidant activity, total phenolics and flavonoid contents of some edible green seaweeds from northern coasts of the Persian Gulf
Iran. J. Pharm. Res. IJPR
Chemical composition, iron bioavailability, and antioxidant activity of Kappaphycus alvarezzi (Doty)
J. Agricult. Food Chem.
Chemical and physico-chemical characterisation of fibres from Laminaria digitata (kombu breton): A physiological approach
J. Sci. Food Agric.
The red seaweed Gracilaria gracilis as a multi products source
Mar. Drugs
Antioxidants versus Food Antioxidant Additives and Food Preservatives
Antioxidants (Basel)
Nutritional Composition and Bioactive Compounds of Red Seaweed: A mini-review
J. Food Nutr. Res.
Marine carotenoids against oxidative stress: effects on human health
Mar. Drugs
Marine Products for Healthcare: Functional and Bioactive Nutraceutical Compounds from the Ocean, Vazhiyil Venugopal: Functional Foods and Nutraceuticals Series
Bioactive compounds in seaweed: functional food applications and legislation
J. Appl. Phycol.
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Co-first author.
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Current affiliation: School of Engineering and Built Environment Kessels Road, Griffith University, QLD - Australia.