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Enterocins as Novel Feed Additives in Rabbit Diet: Enterocin Ent M and Durancin Ent ED26E/7, Their Combination, and Effects on Microbiota, Caecal Fermentation, and Enzymatic Activity

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Abstract

The present study investigates the effects of enterocin Ent M and durancin Ent ED26E/7 applied separately and in combination on the intestinal microbiota, caecal enzymatic activity, and fermentaion of rabbits. Eighty rabbits (M91 meatline, aged 5 weeks, both sexes) were divided into groups E (Ent M; 50 µL/animal/day), D (Ent ED26E/7; 50 µL/animal/day), E + D (Ent M + Ent ED26E/7), and control (C). The additives were administered in drinking water for 21 days. Antimicrobial activity of Ent M and Ent ED26E/7 on coliforms (E, E + D: P < 0.001) and pseudomonads (D: P < 0.05) in feces was noted, compared to C. Ent M and Ent ED26E/7 application stimulated caecal enzymatic activity in rabbits. Pectinolytic (E vs. D, E + D: P < 0.01), inulolytic (E vs. E + D: P < 0.01; E vs. C: P < 0.05), and amylolytic (E vs. D, E + D. P < 0.001; E vs. C: P < 0.01) activities were influenced by Ent M, while cellulolytic (D vs. E + D: P < 0.01) and inulolytic (D vs. E + D, C: P < 0.01) activities by Ent ED26E/7 treatment. The cellulolytic and pectinolytic acitivities changed with time. Treatment × time interaction was detected for cellulose and xylan degradation. During Ent M and Ent ED26E/7 treatment, increased ammonia, lactic, butyric and iso-valeric acid, and lower acetic, propionic, iso-butyric, valeric, and caproic acid concentrations were noted. It can be concluded that Ent M and Ent ED26E/7 application can improve rabbit health due to reduced spoilage microbiota and enhanced caecal enzymatic activity.

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Acknowledgements

We are grateful to Mrs. M. Bodnárová and Mr. P. Jerga for their skilful technical assistance and also to Dr. V. Párkányi, Dr. R. Jurčík, and Dr. Ľ. Ondruška from the National Agricultural and Food Centre in Nitra for caecal sampling and Mr. J. Pecho for slaughtering.

Funding

This work was supported by the Slovak Grant Agency VEGA, grunt numbers 2/0006/17 and 2/0005/21.

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Authors and Affiliations

  1. Institute of Animal Physiology, Centre of Biosciences of the Slovak Academy of Sciences, Šoltésovej 4-6, 04001, Košice, Slovakia

    M. Pogány Simonová, A. Lauková, A. Kandričáková, J. Ščerbová & V. Strompfová

  2. Department of Animal Nutrition, National Agricultural and Food Centre, Hlohovecká 2, 95141, Nitra-Lužianky, Slovakia

    Ľ. Chrastinová

  3. The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences, Instytucka 3, 05 110, Jabłonna, Poland

    R. Miltko & G. Belzecki

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  1. M. Pogány Simonová
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Contributions

AL: Conceptualization, Data curation, Funding acquisition, Investigation, Project administration, Resources, Validation, Writing—review and editing. ĽCh: Data curation, Methodology. AK, JŠ, VS, RM, GB: Methodology. MPS: Project administration, Writing—original draft preparation, Writing—review and editing.

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Correspondence to M. Pogány Simonová.

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Pogány Simonová, M., Lauková, A., Chrastinová, Ľ. et al. Enterocins as Novel Feed Additives in Rabbit Diet: Enterocin Ent M and Durancin Ent ED26E/7, Their Combination, and Effects on Microbiota, Caecal Fermentation, and Enzymatic Activity. Probiotics & Antimicro. Prot. 13, 1433–1442 (2021). https://doi.org/10.1007/s12602-021-09809-0

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