Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
Volume 1868, Issue 9, August 2021, 119078
Light chain subunit of a poorly soluble human IgG2λ crystallizes in physiological pH environment both in cellulo and in vitro
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Abbreviations
CDR
complementarity-determining region;
DIC
differential interference contrast
ER
endoplasmic reticulum
Fab
antigen binding fragment
Fc
crystallizable fragment
HEK
human embryonic kidney
HC
heavy chain
IF
immunofluorescent
Ig
immunoglobulin
mAb
monoclonal antibody
LC
light chain
LLPS
liquid-liquid phase separation
PBS
phosphate-buffered saline
SDS-PAGE
sodium dodecyl sulfate polyacrylamide gel electrophoresis
SEC
size-exclusion chromatography
TBS
Tris-buffered saline
UPR
unfolded protein response
VH
heavy variable region
VL
light chain variable domain
Keywords
Immunoglobulin
Inclusion body
Protein phase separation
Intracellular protein crystallization
Endoplasmic reticulum
Light chain
Antibody solubility
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© 2021 Amgen Inc. Published by Elsevier B.V.