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Promotion effect of the propolis from Jeju Island, Korea, on NGF secretion in human glioblastoma cells

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Abstract

Abstract

Propolis is a resinous mixture of substances collected and processed from various botanical sources by honeybees (Apis mellifera). We previously found that propolis collected on Jeju Island, located off the southern coast of Korea, originates from a single plant, Angelica keiskei KOIDZUMI (Ashitaba). A. keiskei has been well-studied as a health food and has been reported to promote nerve growth factor (NGF) production. Propolis formed from the resin of A. keiskei is expected to have a similar promotional effect on NGF production. NGF is a potential pharmacological agent for Alzheimer’s disease. In this study, the effects of an ethanolic extract of propolis from Jeju Island (EEPJ) on NGF secretion and cell viability in T98G human glioblastoma cells were evaluated. Ethanolic extracts of propolis from Brazil (Baccharis type) and from Uruguay (Populus type) were also studied for comparison. We found that EEPJ significantly increased NGF secretion in the cells in a concentration-dependent manner. Furthermore, the effects of 27 compounds previously isolated from EEPJ were also evaluated. Several compounds were found to have a promotion effect on NGF secretion, and the structure–activity relationships of the compounds were considered relative to their promotional effect on NGF biosynthesis. The promotional effect of EEPJ is a characteristic biological activity that is not present with other propolis types, so the propolis from Jeju Island may have potential applications as a therapeutic candidate for Alzheimer’s disease.

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Funding

This study was funded by JSPS KAKENHI grant numbers 23580180 and 18KK0165.

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Authors and Affiliations

  1. Department of Food and Nutritional Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, Shizuoka, 422-8526, Japan

    Kohsuke Shimomura, Hironari Kako, Hidehiko Yokogoshi & Shigenori Kumazawa

  2. Department of Food Science and Nutrition, College of Health Sciences, Dong-A University, Busan, 49315, Republic of Korea

    Mok-Ryeon Ahn

Authors
  1. Kohsuke Shimomura
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  2. Hironari Kako
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  3. Hidehiko Yokogoshi
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  4. Mok-Ryeon Ahn
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  5. Shigenori Kumazawa
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Correspondence to Shigenori Kumazawa.

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Shimomura, K., Kako, H., Yokogoshi, H. et al. Promotion effect of the propolis from Jeju Island, Korea, on NGF secretion in human glioblastoma cells. J Nat Med 75, 1030–1036 (2021). https://doi.org/10.1007/s11418-021-01535-9

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  • DOI: https://doi.org/10.1007/s11418-021-01535-9

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