Abstract
Aluminum (Al) toxicity is a major limitation to crop production in countries where acidic soil is abundant. In China, soybean production is constrained by Al stress-induced toxicity. As such, there is growing interest to develop Al-resistant varieties. In the present study, we sought to determine potential genes, functions and pathways for screening and breeding of Al-resistant varieties of soybean. First, we mined the E-GEOD-18517 dataset and identified 729 differentially expressed genes (DEGs) between untreated and Al-treated groups. Next, we performed Gene Ontology and Kyoto Encyclopedia of Genes and Genome pathways enrichment analysis and observed that most of the screened genes were mainly enriched in defense response, plasma membrane and molecular transducer activity. They were also enriched in three important pathways, the phenylpropanoid biosynthesis, plant-pathogen interaction, and cutin, suberine and wax biosynthesis. Utilizing weighted gene co-expression network analysis of 815 DEGs screened by Venn diagram, we identified DEGs that were the most disparate between treated and untreated groups. LOC100793667 (probable protein phosphatase 2C 60, GLYMA_17G223800), LOC100780576 (ethylene-responsive transcription factor 1B, GLYMA_02G006200), and LOC100785578 (protein ESKIMO 1, GLYMA_02G258000) were the most differentially expressed, which were consistent with the qRT-PCR results. As these genes are known to participate in essential functions, such as cell junction and phenylpropanoid biosynthesis, these genes may be important for breeding Al-resistant varieties.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Data and availability
All data generated or analyzed during this study are included in this published article.
References
Bahieldin A et al (2016) Ethylene responsive transcription factor ERF109 retards PCD and improves salt tolerance in plant. BMC Plant Biol 16:216. https://doi.org/10.1186/s12870-016-0908-z
Cai WQ, Li HQ, Zhang YB (2004) Effect of Al3+ concentration on the properties of boehmites synthesized by hydrothermal homogeneous precipitation method. Gongneng Cailiao/J Func Mater 35:779–781
Cohen MP (2002) Albumin-binding compounds that prevent nonenzymatic glycation and that may be used for treatment of glycation-related pathologies. US, US6355680 B1
Fukao T, Xu K, Ronald PC, Bailey-Serres J (2006) A variable cluster of ethylene response factor-like genes regulates metabolic and developmental acclimation responses to submergence in rice. Plant Cell 18:2021–2034. https://doi.org/10.1105/tpc.106.043000
Hargrove WL, Thomas GW (1981) Effect of organic matter on exchangeable aluminum and plant growth in acid soils. Chem Soil Environ. https://doi.org/10.2134/asaspecpub40.c810.2134/asaspecpub40.c8
Haug A, Foy CE (1984) Molecular aspects of aluminum toxicity Crit Rev. Plant Sci 1:345–373. https://doi.org/10.1080/07352688409382184
Hou NN, You JF, Pang JD, Xu MY, Chen G, Yang ZM (2010) The accumulation and transport of abscisic acid insoybean (Glycine max L.) under aluminum stress. Plant Soil 330:127–137
Javed AM, Rincon-Zachary M (2006) In vitro and in vivo studies of the effects of aluminum on wheat (Triticum aestivum L.) root cell wall peroxidases. Texas J Sci 58:119–140
Kagawa T, Kadota A, Wada M (1992) The junction between the plasma membrane and the cell wall in fern protonemal cells, as visualized after plasmolysis, and its dependence on arrays of cortical microtubules. Protoplasma 170:186–190. https://doi.org/10.1007/BF01378793
Kochian LV (1995) Cellular mechanisms of aluminum toxicity and resistance in plants. Annu Rev Plant Physiol Plant Mol Biol 46:237–260
Li W, Sun Y, Wang B, Xie H, Wang J, Nan Z (2020) Transcriptome analysis of two soybean cultivars identifies an aluminum respon-sive antioxidant enzyme GmCAT1. Biosci Biotechnol Biochem. https://doi.org/10.1080/09168451.2020.1740970
Matsoukas IG, Massiah AJ, Thomas B (2012) Florigenic and antiflorigenic signaling in plants. Plant Cell Physiol 53:1827–1842. https://doi.org/10.1093/pcp/pcs130
Meskiene I, Bögre L, Glaser W, Balog J, Brandstötter M, Zwerger K, Ammerer G, Hirt H (1998) MP2C, a plant protein phosphatase 2C, functions as a negative regulator of mitogen-activated protein kinase pathways in yeast and plants. Proc Natl Acad Sci U S A 95:1938–1943. https://doi.org/10.1073/pnas.95.4.1938
Miller JB, Pratap A, Miyahara A, Zhou L, Bornemann S, Morris RJ, Oldroyd GE (2013) Calcium/Calmodulin-dependent protein kinase is negatively and positively regulated by calcium, providing a mechanism for decoding calcium responses during symbiosis signaling. Plant Cell 25:5053–5066. https://doi.org/10.1105/tpc.113.116921
Panda SK, Baluska F, Matsumoto H (2009) Aluminum stress signaling in plants. Plant Signal Behav 4:592–597. https://doi.org/10.4161/psb.4.7.8903
Panhwar QA, Naher UA, Radziah O, Shamshuddin J, Razi IM (2015) Eliminating aluminum toxicity in an acid sulfate soil for rice cultivation using plant growth promoting bacteria. Molecules (Basel, Switzerland) 20:3628–3646. https://doi.org/10.3390/molecules20033628
Peter N (1957) Some effects of calcium on the absorption of other ions by plants grown in different soils. University of Oxford. https://ethos.bl.uk/OrderDetails.do?uin=uk.bl.ethos.671884
Reyes D, Rodríguez D, González-García MP, Lorenzo O, Nicolás G, García-Martínez JL, Nicolás C (2006) Overexpression of a protein phosphatase 2C from beech seeds in Arabidopsis shows phenotypes related to abscisic acid responses and gibberellin biosynthesis. Plant Physiol 141:1414–1424. https://doi.org/10.1104/pp.106.084681
Ruhland CT (2001) Effects of ultraviolet-B radiation on phenylpropanoids and growth of vascular plants in Antarctica. Arizona State University
Weihe E, Hartschuh W, Metz J, Brühl U (1977) The use of ionic lanthanum as a diffusion tracer and as a marker of calcium binding sites. Cell Tissue Res 178:285–302. https://doi.org/10.1007/bf00218693
Wiener J, Spiro D, Loewenstein WR (1964) Studies on an epithelial (gland) cell junction. II. Surf Struct J Cell Biol 22:587–598. https://doi.org/10.1083/jcb.22.3.587
Wu D, Ji J, Wang G, Guan C, Jin C (2014) LchERF, a novel ethylene-responsive transcription factor from Lycium chinense, confers salt tolerance in transgenic tobacco. Plant Cell Reports 33:2033–2045. https://doi.org/10.1007/s00299-014-1678-4
Yokel RA, Florence RL (2006) Aluminum bioavailability from the approved food additive leavening agent acidic sodium aluminum phosphate, incorporated into a baked good, is lower than from water. Toxicology 227:86–93. https://doi.org/10.1016/j.tox.2006.07.014
Acknowledgements
We thank professor Yi Zheng of BUA Bioinformatics Center for his contribution to this article.
Funding
The work was partly supported by Research Fund for Academic Degree & Graduate Education of Beijing University of Agriculture; 2020–2022 youth talent promotion project of Beijing Association For Science and Technology and General Project of Science and Technology Program of Beijing Education Commission [KM201910020003]; Research Fund for Academic Degree & Graduate Education of Beijing University of Agriculture [2021YJS046].
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Conflict of interest
The authors declare that they have no conflict of interest.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Wang, Jx., Wang, B., Cui, Lb. et al. Identification of differentially expressed genes associated with aluminum resistance in the soybean plant. Physiol Mol Biol Plants 27, 1311–1321 (2021). https://doi.org/10.1007/s12298-021-01018-x
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12298-021-01018-x