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The Golgi apparatus is the main microtubule-organizing center in differentiating skeletal muscle cells

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Abstract

Studies in differentiating skeletal muscle cells in vitro have revealed that the microtubule-organizing center shifts from the centrosome to the perinuclear sites. As the Golgi apparatus surrounds the nucleus in a myotube, it is unclear whether microtubules are nucleated at the nuclear envelope or at the surrounding Golgi apparatus. In this study, we investigated the positional relationship between the microtubule nucleating sites and the Golgi apparatus in C2C12 myotubes and in primary cultured mouse skeletal myotubes. We focused on gaps in the perinuclear Golgi apparatus where the nuclear envelope was not covered with the Golgi apparatus. In microtubule regrowth assay, microtubule regrowth after cold-nocodazole depolymerization of preexisting microtubules was not found at the gap of the perinuclear Golgi apparatus. Most of the microtubule regrowth was detected at the CDK5RAP2 (CDK5 regulatory subunit-associated protein 2)-rich spots on the perinuclear Golgi apparatus. Disruption of the perinuclear Golgi apparatus with brefeldin A treatment eliminated the perinuclear microtubule regrowth. The Golgi apparatus of undifferentiated myoblasts and those at the cytoplasm of myotubes were also the microtubule nucleating sites. From these observations, we concluded that most of the perinuclear microtubule nucleation occurs on the Golgi apparatus surrounding the nucleus.

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Availability of data and material

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

This study was funded by the Japanese Society for the Promotion of Science (JSPS KAKENHI grant number JP17K07083).

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Authors and Affiliations

  1. Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University, 7-1 Kioicho, Chiyoda-ku, Tokyo, Japan

    Koyo Ide, Mika Muko & Kensuke Hayashi

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  1. Koyo Ide
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  2. Mika Muko
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  3. Kensuke Hayashi
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Contributions

KH designed and supervised the study. YI performed the analysis on C2C12 cells, and MM performed the analysis on primary cultured muscle cells. All authors read and approved the final manuscript.

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Correspondence to Kensuke Hayashi.

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The authors declare no potential conflicts of interest with respect to the research, authorship and publication of this article.

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Ide, K., Muko, M. & Hayashi, K. The Golgi apparatus is the main microtubule-organizing center in differentiating skeletal muscle cells. Histochem Cell Biol 156, 273–281 (2021). https://doi.org/10.1007/s00418-021-01999-6

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