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RpoS Activates the Prodigionsin Production by Activating the Transcription of the RpoS-Dependent Pig Gene Cluster in Serratia marcescens FS14

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Abstract

RpoS, an alternative sigma factor of RNA polymerase, regulates the expression of a great deal of genes involved in stationary-phase survival and stress response. To identify the function of RpoS homologue in Serratia marcescens FS14, in-frame deletion mutant of rpoS was constructed. It was found that RpoS activates the biosynthesis of prodigiosin in FS14 which is just opposite to what was observed in Serratia sp. ATCC 39006. We also demonstrated that RpoS positively regulates the prodigiosin production by activating the transcription of pig cluster in FS14, and the transcription of pig cluster is RpoS-dependent. Further study showed that the differences in the promoters of pig clusters in FS14 and 39006 lead to the different selection of the sigma factors and result in the different regulation mechanisms. The -10 element and the spacer region between -10 and -35 elements of the pig cluster in FS14 are vital for the RpoS recognition in FS14.

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The genome data had been deposited into NCBI GenBank with the Accession number: CP005927.

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Acknowledgements

This work was supported by Grant Nos. 31770050, 31770074 from the National Natural Science Foundation of China.

Funding

This work was supported by Grant Nos. 31770050, 31770074 from the National Natural Science Foundation of China.

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Authors and Affiliations

  1. Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, China

    Baoling Yang, Fenglian Chu, Haixia Li, Weiwu Wang, Tingting Ran & Dongqing Xu

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  1. Baoling Yang
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  2. Fenglian Chu
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  3. Haixia Li
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  4. Weiwu Wang
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Contributions

BY: Investigation, Data Curation, Visualization, Writing-original draft; FC and HL: Investigation, Formal analysis; WW: Supervision, Funding acquisition, Writing-review & editing; TR: Formal analysis; DX: Conceptualization, Resources, Supervision, Project administration, Funding acquisition, Writing-review & editing.

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Correspondence to Dongqing Xu.

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Yang, B., Chu, F., Li, H. et al. RpoS Activates the Prodigionsin Production by Activating the Transcription of the RpoS-Dependent Pig Gene Cluster in Serratia marcescens FS14. Indian J Microbiol 61, 355–363 (2021). https://doi.org/10.1007/s12088-021-00952-4

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