Abstract
Modifying enzyme-CrpP and its variants reduced the MICs of fluoroquinolones in Pseudomonas aeruginosa. This study investigated the dissemination and functional characteristics of CrpP-like in P. aeruginosa from China. The positive rate of crpP-like genes in 228 P. aeruginosa was 25.4% (58/228), and 6 new crpP-like genes were determined. Transformation experiments showed that CrpP-like had a low effect on CIP and LEV susceptibility. The genetic of crpP-positive was diverse. Furthermore, the mean expression level of crpP was no statistical difference between fluoroquinolone-susceptible and -resistant group (P > 0.05). CrpP-like may not play a significant role in fluoroquinolone resistance in P. aeruginosa.
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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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We sincerely thank all the medical workers of the Department of Clinical Laboratory, the First Affiliated Hospital of Wenzhou Medical University for cooperating this study.
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Ye Xu, Ying Zhang, Xiangkuo Zheng, and Kaihang Yu contributed to the acquisition and analysis of the study data. Ye Xu wrote the initial draft of this paper. Yao Sun, Wenli Liao, Huaiyu Jia, and Chunquan Xu performed the analyses. Tieli Zhou and Mo Shen contributed to the concept of the study, the revision of this paper, and the final approval of the version to be published. All authors have read and approved the final manuscript.
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Xu, Y., Zhang, Y., Zheng, X. et al. The prevalence and functional characteristics of CrpP-like in Pseudomonas aeruginosa isolates from China. Eur J Clin Microbiol Infect Dis 40, 2651–2656 (2021). https://doi.org/10.1007/s10096-021-04287-2
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DOI: https://doi.org/10.1007/s10096-021-04287-2