Review articleGenetic engineering strategies for performance enhancement of bioelectrochemical systems: A review
Graphical abstract
Introduction
Depletion of fossil fuels causes energy shortage and environmental problems, suggesting establishing sustainable alternative energy sources. A bioelectrochemical system (BES) is an innovative technology that provides an inexpensive and ecofriendly approach to meet the future energy needs [1]. In fact, the scope of BES goes beyond just energy production to wastewater treatment, bioremediation, production of industrially important chemicals, biosensors and resource recovery [2], [3].
BES can be broadly categorized into various types (microbial fuel cell (MFC), microbial electrolysis cell (MEC), microbial desalination cell (MDC), microbial electrosynthesis cell (MESC) and microbial solar cell (MSC)) depending on their application. However, scalable performance remains the bottleneck towards commercial application of BES. Innovative BES reactor configuration, electrode modification, isolation of novel electroactive microbes and genetic engineering of microbial catalyst are studied to address this problem. Several research groups have investigated on electron transfer pathways of model electroactive microbes and on synthetic biology strategies for enhancing extracellular electron transfer (EET) of microorganisms [4], [5], [6], [7], [8]. Physical, chemical and genetic engineering strategies have been summarized for improving the metabolic functionalities of electroactive microbes in MFC and MESC [9]. Extensive survey of literature revealed that most of the reports focused only on the application of synthetic biology for one specific type of BES i.e. MFC or one particular type of genetic modification strategy. Further, the reactor design of BES, modification of electrode material, various separation membrane and novel cathodic catalyst have been discussed in the recent reviews [10], [11], [12], [13]. Since, the scope of BES is much more than harvesting electrical energy alone in MFC, a critical review on the BES type specific genetic engineering strategies is needed to identify the specific gene modification targets for the development of particular type of BES.
This review will briefly discuss various types of BES and EET pathways of model electroactive microbes; then critically review the genetic engineering approaches for enhancement of BES performance. The main objective of this review is to highlight successful gene modification strategies for a particular type of BES application and limitations of such strategies. BES application where genetic engineering strategies have been less explored and holds potential have been identified. By discussing the advances of gene modification strategies for a specific type of BES application herein, the future prospects of genetic engineering strategies in the development of specific BES type is discussed.
Section snippets
Bioelectrochemical system
By definition, BES is an “electrochemical device that uses microbial catalyst capable of electron exchange with electrode”. Microbes that can release electrons to extracellular acceptors are exoelectrogens and microbes that accept electrons from external donors are electrotrophs [14]. Fig. 1 illustrates the schematic representation of BES reactor along with microbe-electrode interaction. Anodic chamber consists of exoelectrogenic microbes that can oxidize the organics in anolyte and cathodic
Extracellular electron transfer in electroactive microbes
The first ever report on electrical activity of microbes came from measuring the galvanic potential generated by two microbial species i.e., Saccharomyces cerevisiae and Escherichia coli while degrading organic substrates [29]. Although the idea of generating electricity through microbial metabolism is more than 100 years old, any development in BES came only after the discovery of electroactive microbes capable of generating viable current densities. Shewanella and Geobacter species were
Genetically engineered bioelectrochemical system
The limitations of microbial catalyst in BES have been discussed in the above section. Strategies for enhancement of BES performance like physical and chemical pretreatment of microbial cells, entrapment of electroactive microbes over electrode surface and application of electrochemical potential or magnetic field for enriching electroactive communities have been widely adopted [9]. Genetic engineering makes it possible to augment EET rate of microbial catalyst in BES by editing genes to
Critical review
Genetic engineering has been extensively used for studying the EET mechanism of model electroactive microbes and to identify the key components of EET. Multiple genes have been targeted by genetic engineering to either enhance EET or augment the metabolic capacity of the microbial catalyst in BES. It is essential to identify the promising gene modification targets based on BES application and the microbial catalyst used in specific BES. The promising strategies for different type of BES along
Conclusion and future perspective
Understanding the EET pathways of model electroactive microbes have enabled researchers to successfully overexpress these pathways in wild type electroactive microbes as well as extend this property to industrially important non-electroactive microbes using synthetic biology approaches. The metabolic capacity of the microbial catalyst in BES has been extrapolated to make microbial factories for novel substrate oxidation or product synthesis. However, all the successful genetic engineering
CRediT authorship contribution statement
Parini Surti: Conceptualization, Data curation, Writing - original draft. Suresh Kumar Kailasa: Supervision, Writing - review & editing. Arvind Kumar Mungray: Funding acquisition, Supervision, Writing - review & editing.
Declaration of Competing Interest
The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
Acknowledgement
Grant received from SERB-DST, Government of India (File No. EEQ/2016/000802) to carry out this work is duly acknowledged.
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2022, Bioresource TechnologyCitation Excerpt :The choice of electrodes can boost the system performance and shape the microbial community in a bioelectrochemical system. QS is defined as the intracellular microbial communication to control and manipulate biofilm formation (Das et al., 2022; Surti et al., 2021). The microorganisms release self-synthesized extracellular chemical signals called autoinducers, enabling cell–cell communication (Fig. 3B).