Aptamer-Facilitated Design of Gold Nanoparticle-Based Logic Gates for Cyromazine and Melamine Detection in Milk
Haibo Xing A D , Xuelian Fei A D , Bowen Zheng A , Xiyin Zheng B , Xu Dang A , Hongbo Zhang A , Fuxiang Tian A , Xiang Mei C E and Xiaojun Hu
A E
A School of Chemical and Environmental Engineering, Shanghai Institute of Technology, Fengxian District, Shanghai 201418, China.
B Putuo District Center for Disease Control and Prevention, Putuo District, Shanghai 200333, China.
C Wenbo College, East China University of Political Science and Law, Songjiang District, Shanghai 201620, China.
D These authors contributed equally to this work.
E Corresponding authors. Email: kunjia329@126.com; 267358918@qq.com
Australian Journal of Chemistry 74(8) 574-583 https://doi.org/10.1071/CH21011
Submitted: 8 January 2021 Accepted: 2 May 2021 Published: 1 June 2021
Abstract
In this paper, we developed a simply designed detection method for logic gates by using aptamers, cetyltrimethyl ammonium bromide, together with melamine and cyromazine to control the aggregation and dispersion of gold nanoparticles (AuNPs). First, either melamine or cyromazine can induce the aggregation of AuNPs, supporting an OR gate to detect whether they were present or not. Second, based on the fact that aptamer T31 can specifically bind with melamine, preventing the aggregation of AuNPs, an improved INHIBIT gate was also fabricated to find whether there was melamine. It has a detection limit of 0.12 ppm by the naked eye for the detection of melamine, and the limit of detection (LOD) by spectrophotometer is 2.2 ppb. Third, with the adsorption of aptamer Tcy1 on AuNPs and the strong coordination of Tcy1 with cyromazine, the addition of cyromazine and CTAB immediately resulted in the aggregation of AuNPs, giving rise to an AND gate. This gate has a detection limit of 0.17 ppm by the naked eye and the limit of detection (LOD) is 9.0 ppb by spectrophotometer. The system provided a good platform for the development of functional logic systems.
Keywords: aptamer, biosensor, cyromazine, detection, logic gate, melamine, milk.
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