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PeBL1 of Brevibacillus laterosporus a new biocontrol tool for wheat aphid management (Sitobion avenae) in triticum aestivum

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Abstract

The destructive aphid (Sitobion avenae) of wheat, generally accomplished by chemical pesticides, is responsible for the substantial agricultural damages that occur annually. The present experimental study, a protein elicitor, PeBL1, has been examined for the possibility of inducing a defense response to S. avenae in wheat. In comparison to the positive (Water) and negative 70.58 μg ml−1 (50 mM Tris–HCl, pH 8.0) controls, the population growth (intrinsic Increase) rates of S. avenae (second and third generation) with PeBL1-treated wheat seedlings declined. S. avenae preferred control plants for colonizing in an experiment with the host selection compared with the PeBL1-treated wheat plants, with PeBL1 treated wheat seedlings; the nymphal development time of aphid has been extended. In comparison with positive (water) and negative controls, less fecundity was observed with less offspring's in PeBL1-treated wheat seedlings. S. avenae had a harmful surface environment for wheat seedlings treated with PeBL1, caused by trichomes and wax formation. The level of Jasmonic acid (JA), salicylic acid (SA) and ethylene (ET) was significantly higher, and the PeBL1 treated wheat seedlings had significant accumulations. The findings presented that PeBL1 considerably altered the wheat surface structure so that the replication of S. avenae was minimized and colonization was prevented. Defensive processes also include pathway activation (JA, SA, and ET). A biocontrol study for the use of PeBL1 in the protection against S. avenae has been produced in this area.

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Provided in the manuscript file where needed.

Abbreviations

PeBL1:

Protein Elicitor extracted from Brevibacillus laterosporus

JA:

Jasmonic Acid

SA:

Salicylic acid

ET:

Ethylene

AOS :

Coding for allene oxide synthase

PR1 :

Pathogenesis-related protein

ICS :

Isochromate synthase

FAD :

Actin-depolymerizing factor

ADF :

Actin-depolymerizing factor

CYP :

Cytochrome P450 monooxygenase

CHS :

Chalcone synthase

References

  • Ahuja I, Rohloff J, Bones AM (2009) Defence mechanisms of brassicaceae: Implications for plant-insect interactions and potential for integrated pest management. Sustain Agric 2:623–670

    Google Scholar 

  • Ali JG, Agrawal AA (2014) Asymmetry of plant-mediated interactions between specialist aphids and caterpillars on two milkweeds. Funct Ecol 28:1404–1412

    Article  Google Scholar 

  • Ali JG, Agrawal AA (2012) Specialist versus generalist insect herbivores and plant defense. Trends Plant Sci 17:293–302

    Article  CAS  PubMed  Google Scholar 

  • Bale JS, Masters GJ, Hodkinson ID, Awmack C, Bezemer TM, Brown VK, Buse BJ, Coulson A, Farrar JC, Good J, Harrington JEG, Hartley R, Jones S, Lindroth TH, Press RL, Symrnioudis MC, Watt I, AD, Whittaker JB, (2002) Herbivory in global climate change research: Direct effects of rising temperature on insect herbivores. Glob Chang Biol 8:1–16

    Article  Google Scholar 

  • Boughton AJ, Hoover K, Felton GW (2006) Impact of chemical elicitor applications on greenhouse tomato plants and population growth of the green peach aphid. Myzus Persicae Entomol Exp Appl 120:175–188

    Article  CAS  Google Scholar 

  • Boughton AJ, Hoover K, Felton GW (2005) Methyl jasmonate application induces increased densities of glandular trichomes on tomato. Lycopersicon Esculentum J Chem Ecol 31:2211–2216

    Article  CAS  PubMed  Google Scholar 

  • Brogden KA (2005) Antimicrobial peptides: Pore formers or metabolic inhibitors in bacteria? Nat Rev Microbiol 3:238–250

    Article  CAS  PubMed  Google Scholar 

  • De Vos M, Jander G (2009) Myzus persicae (green peach aphid) salivary components induce defence responses in Arabidopsis thaliana. Plant, Cell Environ 32:1548–1560

    Article  Google Scholar 

  • De Vos M, Van Oosten VR, Van Poecke RMP, Van Pelt JA, Pozo MJ, Mueller MJ, Buchala AJ, Métraux JP, Van Loon LC, Dicke M (2005) Signal signature and transcriptome changes of Arabidopsis during pathogen and insect attack. Mol Plant-Microbe Interact 18:923–937

    Article  PubMed  Google Scholar 

  • Derbalah A, Elsharkawy MM, Hamza A, El-Shaer A (2019) Resistance induction in cucumber and direct antifungal activity of zirconium oxide nanoparticles against Rhizoctonia solani. Pestic Biochem Physiol 157:230–236

    Article  CAS  PubMed  Google Scholar 

  • Ellis JG, Rafiqi M, Gan P, Chakrabarti A, Dodds PN (2009) Recent progress in discovery and functional analysis of effector proteins of fungal and oomycete plant pathogens. Curr Opin Plant Biol 12:399–405

    Article  CAS  PubMed  Google Scholar 

  • Farmer EE, Johnson RR, Ryan CA (1992) Regulation of expression of proteinase inhibitor genes by methyl jasmonate and jasmonic acid. Plant Physiol 98:995–1002

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ferrer JL, Jez JM, Bowman ME, Dixon RA, Noel JP (1999) Structure of chalcone synthase and the molecular basis of plant polyketide biosynthesis. Nat Struct Biol 6:775–784

    Article  CAS  PubMed  Google Scholar 

  • Glas JJ, Schimmel BCJ, Alba JM, Escobar-Bravo R, Schuurink RC, Kant MR (2012) Plant glandular trichomes as targets for breeding or engineering of resistance to herbivores. Int j Mol Sci 13:17077–17103

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jakobs R, Schweiger R, Müller C (2019) Aphid infestation leads to plant part-specific changes in phloem sap chemistry, which may indicate niche construction. New Phytol 221:503–514

    Article  CAS  PubMed  Google Scholar 

  • Jarošová J, Kundu JK (2010) Validation of reference genes as internal control for studying viral infections in cereals by quantitative real-time RT-PCR. BMC Plant Biol 10.

  • Javed K, Javed H, Mukhtar T, Qiu D (2019a). Pathogenicity of some entomopathogenic fungal strains to green peach aphid, Myzus persicae Sulzer (Homoptera: Aphididae). Egypt. J. Biol. pest Control 29:

  • Javed K, Javed H, Mukhtar T, Qiu D (2019) Efficacy of beauveria bassiana and verticillium lecanii for the management of whitefly and aphid. Pakistan j Agric Sci 56:669–674

    Google Scholar 

  • Javed K, Qiu D (2020) Protein Elicitor PeBL1 of Brevibacillus laterosporus Enhances Resistance Against Myzus persicae in Tomato. Pathogens 1–21:

  • Javed K, Javed H, Qiu D (2020) Biocontrol Potential of Purified Elicitor Protein PeBL1 Extracted from Brevibacillus laterosporus Strain A60 and Its Capacity in the Induction of Defense Process against Cucumber Aphid (Myzus persicae) in Cucumber (Cucumis sativus). Biology 9:179

    Article  CAS  PubMed Central  Google Scholar 

  • Kots K, Meijer HJG, Bouwmeester K, Govers F, Ketelaar T (2017) Filamentous actin accumulates during plant cell penetration and cell wall plug formation in Phytophthora infestans. Cell Mol Life Sci 74:909–920

    Article  CAS  PubMed  Google Scholar 

  • Lam WKF, Pedigo LP (2009) Effect of Trichome Density on Soybean Pod Feeding by Adult Bean Leaf Beetles (Coleoptera: Chrysomelidae). J Econ Entomol 94:1459–1463

    Article  Google Scholar 

  • Lazebnik J, Frago E, Dicke M, van Loon JJA (2014) Phytohormone Mediation of Interactions Between Herbivores and Plant Pathogens. J Chem Ecol 40:730–741

    Article  CAS  PubMed  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. Methods 25:402–408

    Article  CAS  PubMed  Google Scholar 

  • Li L, Wang S, Yang X, Francis F, Qiu D (2019) Protein elicitor PeaT1 enchanced resistance against aphid (Sitobion avenae) in wheat. Pest Manag Sci 76:236–243

    Article  PubMed  Google Scholar 

  • Mahmoud F, Mahfouz H (2015) Effects of salicylic acid elicitor against aphids on wheat and detection of infestation using infrared thermal imaging technique in Ismailia. Egypt Pesticidii Fitomedicina 30:91–97

    Article  CAS  Google Scholar 

  • Mallinger RE, Hogg DB, Gratton C (2011) Methyl Salicylate Attracts Natural Enemies and Reduces Populations of Soybean Aphids (Hemiptera: Aphididae) in Soybean Agroecosystems. J Econ Entomol 104:115–124

    Article  PubMed  Google Scholar 

  • Montesano M, Brader G, Palva ET (2003) Pathogen derived elicitors: Searching for receptors in plants. Mol Plant Pathol 4:73–79

    Article  CAS  PubMed  Google Scholar 

  • Moran PJ, Thompson GA (2001) Molecular responses to aphid feeding in Arabidopsis in relation to plant defense pathways. Plant Physiol 125:1074–1085

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nouri Ganbalani G, Borzoui E, Shahnavazi M, Nouri A (2018) Induction of resistance against Plutella xylostella (L.) (Lep.: Plutellidae) by jasmonic acid and mealy cabbage aphid feeding in Brassica napus L. Front Physiol 9:1–11

    Article  Google Scholar 

  • Ruiu L, Satta A, Floris I (2013) Emerging entomopathogenic bacteria for insect pest management. Bull Insectology 66:181–186

    Google Scholar 

  • Reeves AF (1977) Tomato trichomes and mutations affecting their development. Am j Bot 64:186–189

    Article  Google Scholar 

  • Saad KA, Mohamad Roff MN, Hallett RH, Idris AB (2015) Aphid-induced Defences in Chilli Affect Preferences of the Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae). Sci Rep 5:1–9

    Article  Google Scholar 

  • Salzman RA, Brady JA, Finlayson SA, Buchanan CD, Summer EJ, Sun F, Klein PE, Klein RR, Pratt LH, Cordonnier-Pratt MM, Mullet JE (2005) Transcriptional profiling of sorghum induced by methyl jasmonate, salicylic acid, and aminocyclopropane carboxylic acid reveals cooperative regulation and novel gene responses. Plant Physiol 138:352–368

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Schaller F, Schaller A, Stintzi A (2004) Biosynthesis and metabolism of jasmonates. J Plant Growth Regul 23:179–199

    Article  CAS  Google Scholar 

  • Schuler MA (1996) The role of cytochrome P450 monooxygenases in plant-insect interactions. Plant Physiol 112:1411–1419

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shenashen M, Derbalah A, Hamza A, Mohamed A, El Safty S (2017) Antifungal activity of fabricated mesoporous alumina nanoparticles against root rot disease of tomato caused by Fusarium oxysporium. Pest Manag Sci 73:1121–1126

    Article  CAS  PubMed  Google Scholar 

  • Thaler JS, Humphrey PT, Whiteman NK (2012) Evolution of jasmonate and salicylate signal crosstalk. Trends Plant Sci 17:260–270

    Article  CAS  PubMed  Google Scholar 

  • Tozin LR, dos S Marques, MOM Rodrigues, TM, (2017) Herbivory by leaf-cutter ants changes the glandular trichomes density and the volatile components in an aromatic plant model. AoB Plants 9:1–8

    Article  Google Scholar 

  • Tian D, Tooker J, Peiffer M, Chung SH, Felton GW (2012) Role of trichomes in defense against herbivores Comparison of herbivore response to woolly and hairless trichome mutants in tomato (Solanum lycopersicum). Planta 236:1053–1066

    Article  CAS  PubMed  Google Scholar 

  • Walling LL (2000) The myriad plant responses to herbivores. J Plant Growth Regul 19:195–216

    Article  CAS  PubMed  Google Scholar 

  • Wang H, Yang X, Guo L, Zeng H, Qiu D (2015) PeBL1, a novel protein elicitor from Brevibacillus laterosporus strain A60, activates defense responses and systemic resistance in Nicotiana benthamiana. Appl Environ Microbiol 81:2706–2716

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wang Y, Sheng L, Zhang H, Du X, An C, Xia X, Chen F, Jiang J, Chen S (2017) CmMYB19 over-expression improves aphid tolerance in chrysanthemum by promoting lignin synthesis. Int j Mol Sci 18:619

    Article  PubMed Central  Google Scholar 

  • Wyatt IJ, White PF (1977) Simple estimation of intrinsic increase rates for aphids and tetranychid mites. J Appl Ecol 14:757–766

    Article  Google Scholar 

  • Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283–333

    Article  CAS  PubMed  Google Scholar 

  • Zhao LY, Chen JL, Cheng DF, Sun JR, Liu Y, Tian Z (2009) Biochemical and molecular characterizations of Sitobion avenae-induced wheat defense responses. Crop Prot 28:435–442

    Article  CAS  Google Scholar 

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Acknowledgements

The authors sincerely thank Prof. Dr. Toby Bruce and Dr. David Buss from Keele University, the UK, for extensive language editing of this manuscript. Kashan Khan helped with data presented in the article. The experiments were carried out in the key Laboratory of Bio pesticides and Engineering, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, P. R. China.

Funding

National Key Research and Development Program of China (2017YFDO200900) supported the study for experimental work.

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KJ planned and designed the experiment; KJ, including paperwork, conducted the experiment. DQ monitored the whole experimental work. HJ and DQ assisted in the analysis of the data. All authors have read and approved the manuscript. The experiment was conducted in the the State Key Laboratory for Bio-Pesticides Engineering of Plant Disease Biocontrol and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Science No. 12 Zhong-Guan-Cun South Street, Beijing 100,081, China.

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Correspondence to Dewen Qiu.

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Javed, K., Javed, H. & Qiu, D. PeBL1 of Brevibacillus laterosporus a new biocontrol tool for wheat aphid management (Sitobion avenae) in triticum aestivum. Int J Trop Insect Sci 42, 535–544 (2022). https://doi.org/10.1007/s42690-021-00569-6

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