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Synthetic Biology: Current State and Applications

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Abstract

This review is devoted to synthetic biology. In this field, molecular biology methods and techniques are applied to develop a predetermined cell behavior based on theoretically constructed regulatory networks using engineering approaches. A cell can be considered the device that performs several types of basic functions. These are sensory, information processing, and response formation functions. Endogenous and exogenous physical or chemical factors that trigger information processing cascades are input signals. These include signaling and metabolic pathways. As a rule, changes in these pathways lead to the modification of the transcriptional profile. The response includes alterations in the metabolic profile followed by physiological changes. Taking this into account, a cell and a computer can be considered analogs. Therefore, the principles of engineering can be applied to natural biological systems in general and genetic circuits in particular. In addition to the main areas of synthetic biology, as well as their principles and methods, specific examples of the application of the synthetic biology approach in medicine and other fields were discussed.

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Funding

The study was supported by the Russian Foundation for Basic Research (project no. 18-29-07046).

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Authors and Affiliations

  1. Moscow State University, 119991, Moscow, Russia

    R. A. Vasilev

  2. Mendeleev University of Chemical Technology of Russia, 125047, Moscow, Russia

    V. Yu. Chernikovich, D. A. Sakharov & M. V. Patrushev

  3. National Research Center Kurchatov Institute, 123182, Moscow, Russia

    R. A. Vasilev, M. A. Evteeva & M. V. Patrushev

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Translated by A. Panyushkina

ADDITIONAL INFORMATION

Vasilev R.A., https://orcid.org/0000-0002-6839-905X; e-mail: ruavasilev@gmail.com

Chernikovich V.Yu., e-mail: victoria.artistcat@gmail.com

Evteeva M.A., e-mail: evteeva_ma@rrcki.ru

Sakharov D.A., https://orcid.org/0000-0001-9333-586X; e-mail: sakharov@muctr.ru

Patrushev M.V., https://orcid.org/0000-0002-4748-9283; e-mail: maxpatrushev@yandex.ru

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Vasilev, R.A., Chernikovich, V.Y., Evteeva, M.A. et al. Synthetic Biology: Current State and Applications. Mol. Genet. Microbiol. Virol. 36, 15–26 (2021). https://doi.org/10.3103/S0891416821010079

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