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Antihypernociceptive effects of Petersianthus macrocarpus stem bark on neuropathic pain induced by chronic constriction injury in rats

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Abstract

Petersianthus macrocarpus (Lecythidaceae) stem bark is traditionally used in West and Central Africa for the treatment of boils and pain. The present study examined the chemical composition of the aqueous and methanolic stem bark extracts of P. macrocarpus by liquid chromatography–electrospray ionization–mass spectrometry (LC–ESI–MS) . Their antinociceptive effect was evaluated using chronic constriction injury (CCI)-induced neuropathic pain in a rat model. On the ninth day post-surgery, the pain perception (allodynia and hyperalgesia) of the animals was assessed after the administration of aqueous and methanolic extracts at the doses of 100 and 200 mg/kg. In addition, the effect of the extracts was evaluated on nitric oxide activity and on the expression of pro-inflammatory cytokines (TNF-α, IL-1β, and NF-κB). The LC–ESI–MS analysis revealed the presence of ellagic acid as the major constituent in the methanol extract. Both extracts at the employed doses (100 and 200 mg/kg), significantly (p < 0.01 and p < 0.001) reduced the spontaneous pain, tactile and cold allodynia, and mechanical hyperalgesia. The methanolic extract used at the dose of 200 mg/kg significantly reduced the nitric oxide level (p < 0.001) and the gene expression levels of NF-κB (p < 0.05) and TNF-α (p < 0.01) in the brain. These data may indicate that stem bark extracts of P. macrocarpus possess a potent anti-hypernociceptive effect on CCI neuropathic pain. The inhibition of the nitric oxide pathway as well as the reduction in NF-κB and TNF-α gene expression in the brain may at least partially contribute to this effect. The results further support the use of this plant by traditional healers in pain conditions.

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Acknowledgements

This work was financially supported by the authors’ personal fund and partially by Centre for Science and Technology of the Non Aligned and Other Developing Countries (NAMS & TCentre). We are grateful to the University of Dschang and Birla Institute of Technology and Science (Pilani campus) for providing all the facilities.

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Authors and Affiliations

  1. Department of Biomedical Sciences, Faculty of Health Sciences, University of Buea, P.O. Box 63, Buea, Cameroon

    Francis Desire Tatsinkou Bomba

  2. Neuropathic Pain Research Laboratory, Department of Pharmacy, Birla Institute of Technology and Science-Pilani, Hyderabad Campus. Jawahar Nagar, Shameerpet Mandal R.R. District, Hyderabad, Telangana, 500078, India

    Francis Desire Tatsinkou Bomba, Gangadhar Matharasala, Ram Kumar Mishra, Madhu Babu Battu, Dharmarajan Sriram & Perumal Yogeeswari

  3. Research Unit of Neuro-Inflammatory and Cardiovascular Pharmacology, Laboratory of Animal Physiology and Phytopharmacology, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon

    Telesphore Benoit Nguelefack & Albert Kamanyi

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  1. Francis Desire Tatsinkou Bomba
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Contributions

FDTB prepared the plant extracts and drafted the manuscript. FDTB, GM, RKM, and MBB collected data. FDTB and TBN conducted the statistical analyses. TBN, DS and PY planned the study. TBN, PY and AK coordinated the study and refined the manuscript for publication. All authors read and approved the final manuscript for publication.

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Correspondence to Francis Desire Tatsinkou Bomba or Perumal Yogeeswari.

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Bomba, F.D.T., Nguelefack, T.B., Matharasala, G. et al. Antihypernociceptive effects of Petersianthus macrocarpus stem bark on neuropathic pain induced by chronic constriction injury in rats. Inflammopharmacol 29, 1241–1253 (2021). https://doi.org/10.1007/s10787-021-00821-y

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