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Exploiting the Acidic Extracellular pH: Evaluation of Streptococcus salivarius M18 Postbiotics to Target Cancer Cells

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Abstract

Previously, we showed that the growth, antibiotic resistance, and biofilm formation properties of the pathogens Pseudomonas aeruginosa and Klebsiella pneumonia were tremendously inhibited by the cell-free supernatant of the oral probiotic Streptococcus salivarius M18. These anti-pathogenic activities of the supernatant were more efficient under acidic conditions. The present approach takes advantage of the acidic nature of the tumor microenvironment to evaluate the effect of the S. salivarius M18 postbiotics on colon cancer cells. In both two-dimensional (2D) and three-dimensional (3D) cell culture models, S. salivarius M18 cell-free supernatant showed anti-cancer actions in the pH conditions mimicking the acidity of the tumor. The inhibitory effect was more prominent when the colon cancer cells have been treated with the cell-free supernatant obtained from the inulin incubated S. salivarius M18. The results of this study point out the potential of the S. salivarius M18 functional probiotic products to be used for targeting low pH environments including the unique acidic microenvironment of tumors.

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Data Availability

All data generated during this study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors sincerely acknowledge Dr. Sreeparna Banerjee (Middle East Technical University) and Dr. Sabahattin Cömertpay (Kahramanmaraş Sütçü İmam University) for sharing cell lines and Dr. Rafig Gurbanov (Bilecik Şeyh Edebali University) for sharing bacterial strains and for providing some reagents used in this study. We also thank Bilecik Şeyh Edebali University, Biotechnology Application and Research Center for the provision of the laboratory facilities.

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  1. Department of Biotechnology, Bilecik Şeyh Edebali University, 11230, Bilecik, Turkey

    Sevinç Karaçam

  2. Biotechnology Application and Research Center, Bilecik Şeyh Edebali University, 11230, Bilecik, Turkey

    Sevinç Karaçam & Sinem Tunçer

  3. Department of Medical Services and Techniques, Vocational School of Health Services, Bilecik Şeyh Edebali University, 11230, Bilecik, Turkey

    Sinem Tunçer

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  1. Sevinç Karaçam
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Correspondence to Sinem Tunçer.

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Karaçam, S., Tunçer, S. Exploiting the Acidic Extracellular pH: Evaluation of Streptococcus salivarius M18 Postbiotics to Target Cancer Cells. Probiotics & Antimicro. Prot. 14, 995–1011 (2022). https://doi.org/10.1007/s12602-021-09806-3

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