Abstract
Members of the Raoultella planticola species isolated from soils contaminated with chemical waste were able to use 2,4-dichlorophenoxyacetic/2,4,5-trichlorophenoxyacetic acid as the sole source of carbon and energy. It was found that cells of strains 33-4ch, 36D, and 36T contained plasmids that were designated as pRP33-4ch, pRP36D and pRP36T, respectively. Restriction fragment length polymorphism (RFLP) analysis showed that pRP33-4ch and pRP36T were probably the same plasmid, while pRP36D contained additional fragments in the restriction profiles. Plasmid elimination showed that the genes for the degradation of chlorophenoxyacetic acids have extrachromosomal localization. Polymerase chain reaction (PCR) demonstrated the absence in the genomes of the studied strains of known initiation genes (tfdA and tftA) for the conversion of chlorophenoxyacetic acids. The identified intermediate metabolites (chlorophenoxyacetic and phenoxyacetic acids and 2-keto-3-methylmuconic semialdehyde) indicated that the strains 33-4ch, 36D, and 36T perform complete dechlorination of the aromatic ring of the substrate with its subsequent meta-cleavage.
Similar content being viewed by others
REFERENCES
Phale, P.S., Shah, B.A., and Malhotra, H., Genes, 2019, vol. 10, no. 8, p. 569. https://doi.org/10.3390/genes10080569
Arora, P.K. and Bae, H., Microb. Cell. Fact., 2014, vol. 13, no. 31. https://doi.org/10.1186/1475-2859-13-31
Don, R.H., Weightman, A.J., Knackmuss, H.J., and Timmis, K.N., J. Bacteriol., 1985, vol. 161, no. 3, pp. 85–90.
Laemmli, C., Werlen, C., and Van der Meer, J.R., Arch. Microbiol., 2004, vol. 181, no. 2, pp. 112–121. https://doi.org/10.1007/s00203-003-0634-4
Zharikova, N.V., Yasakov, T.R., Zhurenko, E.Yu., Korobov, V.V., and Markusheva, T.V., Usp. Sovrem. Biol., 2017, vol. 137, no. 5, pp. 514–528. https://doi.org/10.7868/S0042132417050076
Kumar, A., Trefault, N., and Olaniran, A.O., Crit. Rev. Microbiol., 2016, vol. 42, no. 2, pp. 194–208. https://doi.org/10.3109/1040841X.2014.917068
Enguita, F.J. and Leitao, A.L., Biomed Res. Int., 2013, vol. 2013. https://doi.org/10.1155/2013/542168
Danganan, C.E., Ye, R.W., Daubaras, D.L., Xun, L., and Chakrabarty, A.M., Appl. Environ. Microbiol., 1994, vol. 60, no. 11, pp. 4100–4106.
Daubaras, D.L., Saido, K., and Chakrabarty, A.M., Appl. Environ. Microbiol., 1996, vol. 62, no. 11, pp. 4276–4279.
Zaborina, O., Daubaras, D.L., Zago, A., Xun, L., Saido, K., Klem, T., Nikolic, D., and Chakrabarty, A.M., J. Bacteriol., 1998, vol. 180, no. 17, pp. 4667–4675.
Xun, L. and Wagnon, K.B., Appl. Environ. Microbiol., 1995, vol. 61, no. 9, pp. 3499–3502.
Rice, J.F., Menn, F.-M., Hay, A.G., Sanseverino, J., and Sayler, G.S., Biodegradation, 2005, vol. 16, pp. 501–512. https://doi.org/10.1007/s10532-004-6186-8
Huong, N.L., Itoh, K., and Suyama, K., Microbes Environ., 2007, vol. 22, no. 3, pp. 243–256. https://doi.org/10.1264/jsme2.22.243
Zharikova, N.V., Iasakov, T.R., Bumazhkin, B.K., Patutina, E.O., Zhurenko, E.I., Korobov, V.V., Sagitova, A.I., Kuznetsov, B.B., and Markusheva, T.V., Saudi J. Biol. Sci., 2018, vol. 25, no. 4, pp. 660–671. https://doi.org/10.1016/j.sjbs.2016.02.014
Korobov, V.V., Zhurenko, E.Yu., Zharikova, N.V., Yasakov, T.R., and Markusheva, T.V., Moscow Univ. Biol. Sci. Bull., 2017, vol. 72, no. 4, pp. 201–205. https://doi.org/10.3103/S0096392517040083
Markusheva, T.V., Zhurenko, E.Yu., Galkin, E.G., Korobov, V.V., Zharikova, N.V., and Gafiyatova, L.R., Russ. J. Genet., 2004, vol. 40, no. 11, pp. 1210–1214.
Henriksen, T., Christian, H., Hansen, B., and Jacobsen, C.S., Appl. Environ. Microbiol., 2006, vol. 72, no. 2, pp. 1476–1486. https://doi.org/10.1128/AEM.72.2.1476-1486.2006
Maniatis, T., Fritsch, E. F., and Sambrook, J., Molecular Cloning, Cold Spring Harbor, New York: Cold Spring Harbor Lab. Press, 1982.
Metody opredeleniya mikrokolichestv pestitsidov (Methods for Determination of Trace Amounts of Pesticides), Klisenko, M.A., Ed., Moscow: Meditsina, 1984.
Zharikova, N.V., Markusheva, T.V., Galkin, E.G., Korobov, V.V., Zhurenko, E.Yu., Sitdikova, L.R., Kolganova, T.V., Kuznetsov, B.B., and Turova, T.P., Appl. Biochem. Microbiol., 2006, vol. 42, no. 3, pp. 258–262.
Zharikova, N.V., Yasakov, T.R., Zhurenko, E.Yu., Korobov, V.V., and Markusheva, T.V., Russ. J. Genet., 2018, vol. 54, no. 3, pp. 284–295. https://doi.org/10.1134/S1022795418030171
Kilbane, J.J., Chatterjee, D.K., Karns, J.S., Kellogg, S.T., and Chakrabarty, A.M., Appl. Environ. Microbiol., 1982, vol. 44, no. 1, pp. 72–78. https://doi.org/10.1128/AEM.44.1.72-78.1982
Golovleva, L.A., Pertsova, R.N., Evtushenko, L.I., and Baskunov, B.P., Biodegradation, 1990, vol. 1, no. 4, pp. 263–271.
Boye, K. and Hansen, D., Int. J. Med. Microbiol., 2003, vol. 292, nos. 7–8, pp. 495–503. https://doi.org/10.1078/1438-4221-00228
Drancourt, M., Bollet, C., Carta, A., and Rousselier, P., Int. J. Syst. Evol. Microbiol., 2001, vol. 51, no. 3, pp. 925–932. https://doi.org/10.1099/00207713-51-3-925
Xia, X.S., Smith, A.R., and Bruce, I.J., FEMS Microbiol. Lett., 1996, vol. 144, nos. 2–3, pp. 203–206. https://doi.org/10.1111/j.1574-6968.1996.tb08531.x
Vedler, E., Koiv, V., and Heinaru, A., Gene, 2000, vol. 255, pp. 281–288. https://doi.org/10.1016/s0378-1119(00)00329-2
Balajee, S. and Mahadevan, A., FEMS Microbiol. Lett., 1989, vol. 65, nos. 1–2, pp. 223–228. https://doi.org/10.1111/j.1574-6968.1989.tb03626.x
Balajee, S. and Mahadevan, A., Chem. Unzerer Zeit, 1990, vol. 20, no. 6, pp. 607–617. https://doi.org/10.3109/00498259009046876
Hollender, J., Hopp, J., and Dott, W., Appl. Environ. Microbiol., 1997, vol. 63, no. 11, pp. 4567–4572. https://doi.org/10.1128/AEM.63.11.4567-4572.1997
Korobov, V.V., Zhurenko, E.Yu., Galkin, E.G., Zharikova, N.V., Yasakov, T.R., Starikov, S.N., Sagitova, A.I., and Markusheva, T.V., Microbiology (Moscow), 2018, vol. 87, no. 1, pp. 147–150. https://doi.org/10.1134/S0026261718010101
Funding
The work was carried out within the framework of the state assignment of the Ministry of Education and Science of Russia, no. 075-00326-19-00, on the topic No. AAAA-A18-118022190098-9 with the equipment of the Agidel Center for Collective Use of the Ufa Federal Research Center of the Russian Academy of Sciences.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
Rights and permissions
About this article
Cite this article
Zharikova, N.V., Iasakov, T.R., Zhurenko, E.I. et al. Plasmids of the Chlorophenoxyacetic-Acid Degradation of Bacteria of the Genus Raoultella . Appl Biochem Microbiol 57, 335–343 (2021). https://doi.org/10.1134/S0003683821030157
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0003683821030157