Abstract
It is shown that the catalytic center of Thiocapsa roseopersicina remains active after prolonged treatment with cyanide. It was found that the incubation of cyanide-treated hydrogenase in the presence of beta-mercaptoethanol, ferric iron, and sodium sulfide restored the hydrogenase activity in the hydrogen-oxidation reaction in the presence of methylviologen. The process of activity reconstruction depended on time and reached its maximum value (~ 60%) within 30 min at room temperature. In this case, an absorption band at 420 nm appeared in the absorption spectrum of the hydrogenase, which was present in the native hydrogenase and disappeared after treatment with cyanide; this indicated the reconstruction of iron–sulfur clusters. Thus, instead of growing bacteria in the presence of an iron isotope, one can replace 56Fe with 57Fe in the isolated enzyme, which will allow the use of smaller amounts of 57Fe.
Similar content being viewed by others
REFERENCES
Vignais, P. and Billoud, B., Chem. Rev., 2007, vol. 107, no. 6, pp. 4206–4272.
Lubitz, W., Ogata, H., Rudiger, O., and Reijerse, E., Chem. Rev., 2014, vol. 114, no. 8, pp. 4081–4148.
Greening, C., Biswas, A., Carere, C.R., Jackson, C.J., Taylor, M.C., Stott, M.B., Cook, G.M., and Morales, S.E., ISME J., 2016, vol. 10, no. 3, pp. 761–777.
Shafaat, H.S., Rudiger, O., Ogata, H., and Lubitz, W., Biochim. Biophys. Acta, 2013, vol. 1827, nos. 8–9, pp. 986–1002.
Gogotov, I.N., Zadvornyi, O.A., Zorin, N.A., and Serebryakova, L.T., Fotosinteziruyushchie mikroorganizmy (Photosynthesizing Microorganisms), Gal’chenko, V.F. Ed., Proceedings of the S.N. Vinogradskii Microbiology Institute, Moscow: MAKS Press, 2010, vol. 15, pp. 260–289.
Pandelia, M.E., Nitschke, W., Infossi, P., Giudici-Orticoni, M.T., Bill, E., and Lubitz, W., Proc. Natl. Acad. Sci. U. S. A., 2011, vol. 108, no. 15, pp. 6097–6102.
Lauterbach, L, Wang, H.X., Horch, M., Gee, L.B., Yoda, Y., Tanaka, Y., Zebger, I., Lenz, O., and Cramer, S.P., Chem. Sci., 2015, vol. 6, no. 2, pp. 1055–1060.
Zorin, N.A., Zabelin, A.A., Shkuropatov, A.Ya., and Tsygankov, A.A., J. Inorg. Biochem., 2017, vol. 177, no. 1, pp. 190–197.
Malkin, R. and Rabinowitz, J.C., Biochem. Biophys. Res. Commun., 1966, vol. 23, no. 3, pp. 822–827.
Zorin, N.A., Starodubov, A.S., and Tsygankov, A.A., Appl. Biochem. Microbiol., 2020, vol. 56, no. 2, pp. 149–153.
Parret, K.G., Mehari, T., and Golbeck, J.H., Biochim. Biophys. Acta, 1990, vol. 1015, no. 2, pp. 341–352.
Seefeldt, L.C. and Arp, D.J., J. Bacteriol., 1989, vol. 171, no. 6, pp. 3298–3303.
Hexter, S.V., Chung, M.-W., Vincent, K.A., and Armstrong, F.A., J. Am. Chem. Soc., 2014, vol. 136, no. 29, pp. 10470–10477.
Zorin N.A., Dimon B., Gagnon J., Gaillard J., Carrier P., Vignais P.V., Eur. J. Biochem., 1996, vol. 241, no. 4, pp. 675–681.
Jung, Y.-S., Yu, L., and Golbeck, H., Photosynth. Res., vol. 46, no. 1, pp. 249–255.
Mehari, T., Parret, K.G., Warren, P.V., and Golbeck, J.H., Biochim. Biophys. Acta—Bioenergetics, 1991, vol. 1056, no. 1, pp. 139–148.
Funding
The research was supported by the Russian Science Foundation, grant no. 19-14-00255.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
The authors declare that they have no conflict of interest. This article does not contain any studies involving animals or human participants performed by any of the authors.
Rights and permissions
About this article
Cite this article
Zorin, N.A., Khusnutdinova, A.N., Starodubov, A.S. et al. Reconstruction of HydSL Hydrogenase from Thiocapsa roseopersicina after Cyanide Inhibition. Appl Biochem Microbiol 57, 351–355 (2021). https://doi.org/10.1134/S0003683821030169
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S0003683821030169