Abstract
Citrus canker, caused by Xanthomonas citri subsp. citri (Xcc), is a major bacterial disease responsible for substantial economic losses in citrus-producing areas. To breed transgenic citrus plants with enhanced resistance to citrus canker, two antimicrobial peptide genes, PR1aCB and AATCB, were incorporated into ‘Tarocco’ blood orange (Citrus sinensis Osbeck) plants via co-transformation and sequential re-transformation. The presence of PR1aCB and AATCB in double transgenic plants was confirmed by PCR. The expression of PR1aCB and AATCB in double transformants was demonstrated by quantitative real-time PCR. An in vivo disease resistance assay involving the injection of Xcc revealed that the double transformants were more resistant to citrus canker than the single gene transformants and wild-type plants. An analysis of the bacterial population indicated that the enhanced citrus canker resistance of the double transformants was due to inhibited Xcc growth. These results proved that the pyramiding of multiple genes is a more effective strategy for increasing the disease resistance of transgenic citrus plants than single gene transformations.
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Acknowledgements
This research was supported by the Key-Area Research and Development Program of Guangdong Province (2018B020202009), the Earmarked Fund for China Agriculture Research System (CARS-26). We thank Liwen Bianji, Edanz Editing China (www.liwenbianji.cn/ac) for editing the English text of a draft of this manuscript.
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AP designed the experiments, completed the citrus re-transformation, and wrote the manuscript. JZ and XZ completed the citrus co-transformation. LX analyzed the GUS and GFP activities of the regenerants. YH completed the PCR analysis. TL completed the sequencing analysis. LY evaluated the resistance of the transgenic citrus plants to citrus canker. QL completed the expression analysis. SC analyzed the data.
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Peng, A., Zhang, J., Zou, X. et al. Pyramiding the antimicrobial PR1aCB and AATCB genes in ‘Tarocco’ blood orange (Citrus sinensis Osbeck) to enhance citrus canker resistance. Transgenic Res 30, 635–647 (2021). https://doi.org/10.1007/s11248-021-00245-y
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DOI: https://doi.org/10.1007/s11248-021-00245-y