Abstract
Background
Isoflurane (ISO), with the characteristics of rapid induction and recovery, has become one of the most commonly used inhalation anesthetics in the world.
Objective
The purpose of this study was to explore the biological mechanism of long non-coding RNA taurine up-regulated gene 1 (TUG1) in isoflurane (ISO) induced neurotoxicity through in vitro cell experiments.
Result
ISO exposure inhibited cell viability while promoted ROS generation, cell apoptosis and inflammatory cytokines release in HT22 cells via upregulating long noncoding RNA (lncRNA) TUG1. MiR-15a-5p was a direct target of TUG1 and was reversed regulated by TUG1. Overexpression of miR-15a-5p alleviated neurotoxicity induced by ISO exposure, while downregulation of TUG1 alleviated the neurotoxicity induced by ISO exposure via upregulation of miR-15a-5p.
Conclusion
Downregulation of TUG1 reduced ISO-induced ROS generation, neuron cell apoptosis and inflammatory response. ISO-induced neurotoxicity in HT22 cells was regulated by TUG1/miR-15a-5p axis.
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This work was supported by the National Key Research and Development Program (2018YFC2001901) and the National Natural Science Foundation of China (NO. 81801193).
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FZ and GC conducted most of the experiments and drafted the manuscript. LW contributed to the statistical analysis and manuscript editing. ZF and WM designed this study, edited the manuscript and made the revision. All authors read and approved the final manuscript.
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Faqiang Zhang, Guoqing Chen, Long Wang, Zeguo Feng, Weidong Mi declare that they have no conflicts of interest.
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Zhang, F., Chen, G., Wang, L. et al. LncRNA taurine up-regulated gene 1 participates in isoflurane induced neurotoxicity. Mol. Cell. Toxicol. 17, 347–356 (2021). https://doi.org/10.1007/s13273-021-00141-w
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DOI: https://doi.org/10.1007/s13273-021-00141-w