Abstract
Vanadocene dichloride (VDC), a vanadium containing metallocene dihalide exhibits promising anticancer activity. However, its mechanism of action remains elusive as several diverse targets and pathways have been proposed for its anticancer activity. In this study, we observed that VDC inhibited the proliferation of mammalian cancer cells and induced apoptotic cell death by altering the mitochondrial membrane potential and the expression of bcl2 and bax. Probing further into its anticancer mechanism, we found that VDC caused depolymerization of interphase microtubules and blocked the cells at mitosis with considerable proportion of cells exhibiting monopolar spindles. The reassembly of cold depolymerized microtubules was strongly inhibited in the presence of 10 μM VDC. VDC perturbed the microtubule-kinetochore interactions during mitosis as indicated by the absence of cold stable spindle microtubules in the cells treated with 20 μM VDC. Using goat brain tubulin, we found that VDC inhibited the steady-state polymer mass of microtubules and bound to tubulin at a novel site with a Kd of 9.71 ± 0.19 μM and perturbed the secondary structure of tubulin dimer. In addition, VDC was also found to bind to the mitotic kinesin Eg5 and inhibit its basal as well as microtubule stimulated ATPase activity. The results suggest that disruption of microtubule assembly dynamics and inhibition of the ATPase activity of Eg5 could be a plausible mechanism for the antiproliferative and antimitotic activity of VDC.
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Abbreviations
- VDC:
-
Vanadocene dichloride
- DMSO:
-
Dimethyl sulfoxide
- PBS:
-
Phosphate buffered saline
- SRB:
-
Sulforhodamine B
- MEM:
-
Minimal essential medium
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Acknowledgements
S.M and D.R would like to thank NITC and MHRD, Government of India for the financial support. The authors also thank Dr. M.K. Mathew, National Centre for Biological Sciences, Bangalore for providing access to the CD spectroscopy facility and Mr. Muthukrishnan M, Technical staff, CSIR-CLRI Chennai for the help in acquiring TEM images.
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SM designed and performed the experiments and wrote the manuscript. DR isolated the Eg5 motor domain, performed binding experiments involving Eg5, analyzed the data, and contributed in manuscript preparation. KR provided the resources for this work, contributed in designing the experiments, analyzing the data, and critical writing of the manuscript. All the authors have read and approved the final version of the manuscript.
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Capra hircus tubulin alpha chain Uniprot ID: A0A452F9Q0 and Capra hircus tubulin beta chain Uniprot ID: A0A452FIJ2.
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Mahanty, S., Raghav, D. & Rathinasamy, K. Vanadocene dichloride induces apoptosis in HeLa cells through depolymerization of microtubules and inhibition of Eg5. J Biol Inorg Chem 26, 511–531 (2021). https://doi.org/10.1007/s00775-021-01872-w
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DOI: https://doi.org/10.1007/s00775-021-01872-w