Abstract
Collagens are the main components of human tissues. Various regulatory factors and cytokines may influence expression levels for collagen-encoding genes, and, therefore, contrubite to some collagen-associated pathologies. In this study, we demonstrate regulatory effects of USF1 on expression of genes encoding fibrillar collagen types I, II, and III in osteoblastic Saos-2 and MG-63 cells. An ectopic expression of the human USF1 led to a decrease in both mRNA and protein expression levels of the collagen-encoding genes mentioned above. ADAMTS-3 is a proteinase primarily responsible for the amino-terminal cleavage of type I and type II collagen precursors. The ADAMTS-3 promoter region contains potential binding sites for USF1. Here we show that an overexpression of USF1 lead to a decrease in ADAMTS-3 mRNA and protein expression levels. In co-transfection studies, USF1 negatively regulated ADAMTS-3 promoter activity. Further, in EMSA studies, we showed that USF1 binds to the ADAMTS-3 promoter region. In conclusion, it seems that ADAMTS-3 and USF1 contribute to the regulation of collagen encoding genes in osteosarcoma.
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ACKNOWLEDGMENTS
Saos-2 cells and USF1 expression plasmid were kindly provided by Dr. Kenneth Wann and by Dr. Dipak P. RAMJI (Cardiff, School of Biosciences, Cardiff UK), respectively. MG-63 cells were kindly provided by Dr. Berivan ÇEÇEN, (Dokuzeylül University, Izmir, TURKEY).
Funding
This work was supported by the Scientific and Technological Research Council of Turkey (TUBITAK), Project number; 114Z025.
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Alper, M., Aydemir, T. & Köçkar, F. USF1 Suppresses Expression of Fibrillar Type I, II, and III Collagen and pNP Adamts-3 in Osteosarcoma Cells. Mol Biol 55, 580–588 (2021). https://doi.org/10.1134/S0026893321030031
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DOI: https://doi.org/10.1134/S0026893321030031