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KaAhl, a Novel N-Acylhomoserine Lactonase from Kushneria avicenniae and Attenuated Effect on the Virulence of Erwinia carotovora

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Abstract

N-acylhomoserine lactones (AHLs) are autoinducers found in gram-negative bacteria and are ubiquitous in common plant pathogenic bacteria. N-acylhomoserine lactonase degrades the lactone ring of AHLs to achieve quorum quenching of phytopathogenic virulence genes. In this study, a novel AHL lactonase gene KaAhl was successfully cloned and identified from Kushneria avicenniae strain DSM 23439. KaAhl is encoded a 261-residue polypeptide belonging to the metallo-β-lactamase superfamily. The recombinant enzyme displayed maximum hydrolysis activity of 0.64 U/mg toward N-(3-oxooctanoyl)-L-homoserine lactone substrate at 30°C and pH 8.0. Besides, it had moderate thermal stability but exhibited a high salt tolerance showing approximate 50% relative activity in the presence of 35% concentrations of NaCl. Furthermore, the KaAhl was significantly promoted by Mn2+ and Ni2+, which enhanced the lactonase activity to approximate 100% extent. In addition, it was observed that recombinant Escherichia coli BL21-pET28a-KaAhl producing KaAhl proteins could effectively inhibit the plant pathogenicity of Erwinia carotovora for 72 h, which might have great potential for controlling gram-negative pathogenic bacteria.

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Acknowledgments

This study was supported by grants from the Key Laboratory of Biotic Environment and Ecology Safety in Anhui Province, Innovation Team of Scientific Research Platform in Anhui Universities, Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, Key Laboratory of Biomedicine in Gene Diseases and Health of Anhui Higher Education Institutes.

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  1. Institute of Molecular Biology and Biotechnology and the Research Center of Life Omics and Health, Anhui Provincial Key Laboratory of the Conservation and Exploitation Research of Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu, Anhui, 241000, China

    Yue Su, Mengru Shao, Tianying Li & Ling Lin

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  1. Yue Su
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Su, Y., Shao, M., Li, T. et al. KaAhl, a Novel N-Acylhomoserine Lactonase from Kushneria avicenniae and Attenuated Effect on the Virulence of Erwinia carotovora. Biotechnol Bioproc E 26, 419–426 (2021). https://doi.org/10.1007/s12257-020-0230-3

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