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Testing carbon and nitrogen sources for the in vitro growth of the model lichenized fungus Endocarpon pusillum Hedw.

Published online by Cambridge University Press:  26 May 2021

Oliver L. Mead Open the ORCID record for Oliver L. Mead [Opens in a new window]  and
Cécile Gueidan Open the ORCID record for Cécile Gueidan [Opens in a new window]
Oliver L. Mead*
Affiliation:
Centre for Australian National Biodiversity Research, CSIRO National Research Collections Australia, GPO Box 1700, Canberra, ACT2601, Australia Synthetic Biology FSP, GPO Box 1700, Canberra, ACT2601, Australia
Cécile Gueidan
Affiliation:
Centre for Australian National Biodiversity Research, CSIRO National Research Collections Australia, GPO Box 1700, Canberra, ACT2601, Australia
*
Author for correspondence: Oliver L. Mead. E-mail: Oliver.Mead@csiro.au
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Abstract

To improve the efficiency of isolating and culturing lichen mycobionts, we performed a growth assay on an Australian strain of the soil-crust lichenized fungus Endocarpon pusillum Hedw. This assay determined the preferred nitrogen and carbon sources of the fungus by limiting the available nitrogen or carbon sources to single compounds found in soils, plants and lichen thalli. We found that the non-proteinaceous amino acid, GABA, produced the most growth of all nutrients when provided as the sole nitrogen source but was a poor carbon source. Fructose, glucose, cellobiose and sorbitol produced the most growth of all the carbon sources tested. Ammonium, nitrate and polyamines were poor nutrient sources. These findings correspond with reports of primary metabolite pools in other lichen species and may guide future studies involving growth of recalcitrant lichen mycobionts.

Keywords

aminobutyricGABAgrowthmediumnutritionpolyalcoholpolyolrate
Type
Standard Papers
Information
The Lichenologist , Volume 53 , Issue 3 , May 2021 , pp. 257 - 264
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Copyright
Copyright © The Author(s), 2021. Published by Cambridge University Press on behalf of the British Lichen Society

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