Skip to main content
Log in

An Apparatus for Laser Engineering of Microbiological Systems

  • PHYSICAL INSTRUMENTS FOR ECOLOGY, MEDICINE, AND BIOLOGY
  • Published:
Instruments and Experimental Techniques Aims and scope Submit manuscript

Abstract

A device for laser bioprinting is described, whose principle of operation is based on the transfer of a microscopic amount of gel with living systems from a donor substrate under the action of nanosecond laser pulses to arbitrary (acceptor) substrates. The use of a nanosecond pulsed laser source, a P-shaper lens, and a motorized telescopic system in the device makes it possible to automatically adjust the parameters of the laser action for various tasks. Using the device it is possible to carry out laser printing of microorganisms, cells, and their agglomerates of various sizes and physical properties. The device allows one to select parameters that provide stable printing modes and minimize negative factors that affect the transferred living microorganisms using gels of various viscosities.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.

Similar content being viewed by others

REFERENCES

  1. Koch, L., Kuhn, S., Sorg, H., Gruene, M., Schlie, S., Gaebel, R., Polchow, B., Reimers, K., Stoelting, S., Ma, N., Vogt, P.M., Steinhoff, G., and Chichkov, B., Tissue Eng., Part C, 2010, vol. 16, no. 5, p. 847. https://doi.org/10.1089/ten.tec.2009.0397

    Article  Google Scholar 

  2. Yusupov, V., Churbanov, S., Churbanova, E., Bardakova, K., Antoshin, A., Evlashin, S., Timashev, P., and Minaev, N., Int. J. Bioprint., 2020, vol. 6, no. 3, p. 1. https://doi.org/10.18063/ijb.v6i3.271

    Article  Google Scholar 

  3. Kochetkova, T.V., Zayulina, K.S., Zhigarkov, V.S., Minaev, N.V., Chichkov, B.N., Novikov, A.A., Toshchakov, S.V., Elcheninov, A.G., and Kublanov, I.V., Int. J. Syst. Evol. Microbiol., 2020, vol. 70, no. 2, p. 1192. https://doi.org/10.1099/ijsem.0.003902

    Article  Google Scholar 

  4. Bello, M.G.D., Knight, R., Gilbert, J.A., and Blaser, M.J., Science, 2018, vol. 362, no. 6410, p. 33. https://doi.org/10.1126/science.aau8816

    Article  ADS  Google Scholar 

  5. Minaev, N.V., Shubnyi, A.G., Yusupov, V.I., Tsvetkov, M.Yu., and Bagratashvili, V.N., Prib. Tekh. Eksp., 2018, no. 6, p. 127. https://doi.org/10.1134/S0032816218060101

  6. Yusupov, V.I., Zhigarkov, V.S., Churbanova, E.S., Chutko, E.A., Evlashin, S.A., Gorlenko, M.V., Cheptsov, V.S., Minaev, N.V., and Bagratashvili, V.N., Quantum Electron., 2017, vol. 47, no. 12, p. 1158. https://doi.org/10.1070/QEL16512

    Article  ADS  Google Scholar 

  7. Minaev, N.V., Yusupov, V.I., Churbanova, E.S., Churbanov, S.N., Zhigar’kov, V.I., and Antoshin, A.A., Prib. Tekh. Eksp., 2019, no. 1, p. 153. https://doi.org/10.1134/S0032816219010191

  8. Minaev, N.V., Yusupov, V.I., and Chichkov, B.N., RF Minor Patent 198221, Byull. Izobret., 2020, no. 18.

  9. Zhigar’kov, V.S., Minaev, N.V., and Yusupov, V.I., Prib. Tekh. Eksp., 2020, no. 1, p. 153. https://doi.org/10.31857/S0032816219060326

Download references

ACKNOWLEDGMENTS

The authors thank V. S. Zhigar’kov and K. I. Kachesov for the biotesting results.

Funding

This work was supported by the Ministry of Science and Higher Education within the framework of the State Assignment of the Crystallography and Photonics Federal Research Center of the Russian Academy of Sciences regarding the calibration of the laser system and the Russian Science Foundation grant 20-14-00286 regarding the development of the LIMS technology.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to V. I. Yusupov.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Minaev, N.V., Epifanov, E.O. & Yusupov, V.I. An Apparatus for Laser Engineering of Microbiological Systems. Instrum Exp Tech 64, 464–467 (2021). https://doi.org/10.1134/S0020441221020147

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S0020441221020147

Navigation