Abstract
A device for laser bioprinting is described, whose principle of operation is based on the transfer of a microscopic amount of gel with living systems from a donor substrate under the action of nanosecond laser pulses to arbitrary (acceptor) substrates. The use of a nanosecond pulsed laser source, a P-shaper lens, and a motorized telescopic system in the device makes it possible to automatically adjust the parameters of the laser action for various tasks. Using the device it is possible to carry out laser printing of microorganisms, cells, and their agglomerates of various sizes and physical properties. The device allows one to select parameters that provide stable printing modes and minimize negative factors that affect the transferred living microorganisms using gels of various viscosities.
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REFERENCES
Koch, L., Kuhn, S., Sorg, H., Gruene, M., Schlie, S., Gaebel, R., Polchow, B., Reimers, K., Stoelting, S., Ma, N., Vogt, P.M., Steinhoff, G., and Chichkov, B., Tissue Eng., Part C, 2010, vol. 16, no. 5, p. 847. https://doi.org/10.1089/ten.tec.2009.0397
Yusupov, V., Churbanov, S., Churbanova, E., Bardakova, K., Antoshin, A., Evlashin, S., Timashev, P., and Minaev, N., Int. J. Bioprint., 2020, vol. 6, no. 3, p. 1. https://doi.org/10.18063/ijb.v6i3.271
Kochetkova, T.V., Zayulina, K.S., Zhigarkov, V.S., Minaev, N.V., Chichkov, B.N., Novikov, A.A., Toshchakov, S.V., Elcheninov, A.G., and Kublanov, I.V., Int. J. Syst. Evol. Microbiol., 2020, vol. 70, no. 2, p. 1192. https://doi.org/10.1099/ijsem.0.003902
Bello, M.G.D., Knight, R., Gilbert, J.A., and Blaser, M.J., Science, 2018, vol. 362, no. 6410, p. 33. https://doi.org/10.1126/science.aau8816
Minaev, N.V., Shubnyi, A.G., Yusupov, V.I., Tsvetkov, M.Yu., and Bagratashvili, V.N., Prib. Tekh. Eksp., 2018, no. 6, p. 127. https://doi.org/10.1134/S0032816218060101
Yusupov, V.I., Zhigarkov, V.S., Churbanova, E.S., Chutko, E.A., Evlashin, S.A., Gorlenko, M.V., Cheptsov, V.S., Minaev, N.V., and Bagratashvili, V.N., Quantum Electron., 2017, vol. 47, no. 12, p. 1158. https://doi.org/10.1070/QEL16512
Minaev, N.V., Yusupov, V.I., Churbanova, E.S., Churbanov, S.N., Zhigar’kov, V.I., and Antoshin, A.A., Prib. Tekh. Eksp., 2019, no. 1, p. 153. https://doi.org/10.1134/S0032816219010191
Minaev, N.V., Yusupov, V.I., and Chichkov, B.N., RF Minor Patent 198221, Byull. Izobret., 2020, no. 18.
Zhigar’kov, V.S., Minaev, N.V., and Yusupov, V.I., Prib. Tekh. Eksp., 2020, no. 1, p. 153. https://doi.org/10.31857/S0032816219060326
ACKNOWLEDGMENTS
The authors thank V. S. Zhigar’kov and K. I. Kachesov for the biotesting results.
Funding
This work was supported by the Ministry of Science and Higher Education within the framework of the State Assignment of the Crystallography and Photonics Federal Research Center of the Russian Academy of Sciences regarding the calibration of the laser system and the Russian Science Foundation grant 20-14-00286 regarding the development of the LIMS technology.
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Minaev, N.V., Epifanov, E.O. & Yusupov, V.I. An Apparatus for Laser Engineering of Microbiological Systems. Instrum Exp Tech 64, 464–467 (2021). https://doi.org/10.1134/S0020441221020147
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DOI: https://doi.org/10.1134/S0020441221020147