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Weissella confusa CGMCC 19,308 Strain Protects Against Oxidative Stress, Increases Lifespan, and Bacterial Disease Resistance in Caenorhabditis elegans

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Abstract

The aim of this study was to investigate the antioxidant activity of Weissella confusa CGMCC 19,308 and its influence on longevity and host defense against Salmonella Typhimurium of Caenorhabditis elegans. The CFCS (cell-free culture supernatant) of W. confusa CGMCC 19,308 possessed DPPH radicals, hydroxyl radicals, and superoxide anion scavenging activity. The lifespan of the C. elegans fed W. confusa CGMCC 19,308 was significantly (p < 0.001) longer than that of worms fed Escherichia coli OP50. Moreover, worms fed W. confusa CGMCC 19,308 were more resistant to oxidative stress induced by hydrogen peroxide and S. Typhimurium infection. RNA-seq analysis showed that the most significantly differentially expressed genes (DEGs) in C. elegans fed with W. confusa CGMCC 19,308 were mainly col genes (col-43, col-2, col-40, col-155, col-37), glutathione–S-transferase (GST)-related genes (gst-44, gst-9, gst-17, gst-18, gstk-2), cnc-9 (immune-related gene), and sod-5 (superoxide dismutase). These results indicated that cuticle collagen synthesis, immunity, and antioxidant defense (AOD) system of C. elegans were affected after being fed with W. confusa CGMCC 19,308 instead of E. coli OP50. Our study suggested W. confusa CGMCC 19,308 had the antioxidant activity and could prolong lifespan and enhance the host defense against S. Typhimurium of C. elegans. This study provided new evidences for the W. confusa CGMCC 19,308 as a potential probiotic candidate for anti-aging and anti-bacterial infection.

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Funding

The study presented in the manuscript was funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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  1. Department of Pharmaceutical Microbiology, School of Life Science and Technology, China Pharmaceutical University, Nanjing, 210009, China

    Wenqian Wang, Shipo Li, Xing Heng & Weihua Chu

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W. C. conceived and designed the study. W. W., S. L., and X. H. performed the experiments. W. W. and W. C. analyzed the data and wrote the manuscript with input from all authors.

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Correspondence to Weihua Chu.

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Wang, W., Li, S., Heng, X. et al. Weissella confusa CGMCC 19,308 Strain Protects Against Oxidative Stress, Increases Lifespan, and Bacterial Disease Resistance in Caenorhabditis elegans. Probiotics & Antimicro. Prot. 14, 121–129 (2022). https://doi.org/10.1007/s12602-021-09799-z

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