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Screening for effective microbial consortia against Fusarium wilt of cape gooseberry (Physalis peruviana)

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Abstract

Fusarium oxysporum Schlecht. (Hypocreales: Nectriaceae) is one of the most devastating plant pathogens worldwide, causing vascular wilt in several crops. Management of this disease primarily relies on chemical fungicides and resistant cultivars in high value crops. However, due to the limited efficacy of these methods, alternative control methods are needed. Biological control is a sustainable, safe, and effective alternative, but the use of a single biological control agent (BCA) usually has inconsistent results. The consistency of biocontrol could be enhanced using microbial consortia. In this context, the aim of this work was to select an effective microbial consortium against vascular wilt in cape gooseberry (Physalis peruviana L.) caused by Fusarium oxysporum f. sp. physali, from a mixture of four strains of Trichoderma spp. Pers. (Hypocreales: Hypocraceae) and Bacillus velezensis (Bacillales: Bacillaceae) Bs006. The calculated synergy factor was used as a selection criterion. Then the selected consortium was evaluated in the field and compared to carbendazim. The Trichoderma virens Gl006 and B. velezensis Bs006 consortium showed synergistic activity against vascular wilt under greenhouse and field conditions and efficacy similar to chemical control. These results suggest that Gl006 and Bs006 have a higher potential in controlling Fusarium wilt in cape gooseberry when applied as a consortium compared to separate, single strains.

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Acknowledgements

This work was carried out with the financial support from Ministerio de Agricultura y Desarrollo Rural—MADR through Tv15 and Tv16 transference resources to Corporación Colombiana de Investigación Agropecuaria—AGROSAVIA, Project 687. The pathogenic strain of F. oxysporum f. sp. physali Map5 as well as the biocontrol strains Gl006, Th003, Th034, Th035 and Bs006 from AGROSAVIA Microorganisms Working-Collection were evaluated in the research that gave rise to this paper.

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  1. Centro de Investigación Tibaitatá, Corporación Colombiana de Investigación Agropecuaria – AGROSAVIA, Km 14 vía Bogotá, Mosquera, Colombia

    L. F. Izquierdo-García, A. M. Cotes & C. A. Moreno-Velandia

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  1. L. F. Izquierdo-García
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  2. A. M. Cotes
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  3. C. A. Moreno-Velandia
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AMC, CAMV and LFIG Conceived the research idea, LFIG and CAMV: Carried out the experiments, LFIG: Drafted the manuscript under advice of CAMV. LFIG, CAMV and AMC: Contributed to analyzed data and critical revising and approval the final version of the manuscript.

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Correspondence to L. F. Izquierdo-García.

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Izquierdo-García, L.F., Cotes, A.M. & Moreno-Velandia, C.A. Screening for effective microbial consortia against Fusarium wilt of cape gooseberry (Physalis peruviana). BioControl 66, 713–725 (2021). https://doi.org/10.1007/s10526-021-10095-6

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