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Fabrication and fluorescence imaging properties of indocyanine green-loaded poly(lactic-co-glycolic acid) nanoparticles

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Abstract

This work proposed a method for fabricating poly(lactic-co-glycolic acid) (PLGA) nanoparticles (ca. 70 nm) loaded with indocyanine green (ICG) molecules by an antisolvent crystallization method using dimethyl sulfoxide (DMSO) as a good solvent and water as a poor solvent. ICG molecules were loaded to PLGA nanoparticles by mixing PLGA, polyethylenimine, and polyethylene glycol (PEG) in DMSO with polyvinyl alcohol, albumin, and ICG in water. The ICG-loaded PLGA nanoparticle colloid solutions emitted fluorescence even at high ICG concentrations. Not only the tissues of a tumor-bearing mouse but also the tumor could be imaged with an IVIS system by injecting ICG-loaded PLGA nanoparticle colloid solution into the tail vein of the mouse and measuring the emitted fluorescence intensity, though some ICG-loaded PLGA nanoparticles were trapped in tissues such as the liver and spleen due to insufficient PEGylation. Thus, this work showed that ICG-loaded PLGA nanoparticles have potential for cancer imaging.

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Abbreviations

ICG:

Indocyanine green

PLGA:

Poly(lactic-co-glycolic acid)

AIE:

Aggregation-induced emission

PEI:

Polyethylenimine

DMSO:

Dimethyl sulfoxide

PEG:

Polyethylene glycol

PVA:

Polyvinyl alcohol

BSA:

Bovine serum albumin

TEM:

Transmission electron microscopy

ELS:

Electrophoretic light scattering

UV-VIS-NIR:

Ultraviolet-visible-near-infrared extinction spectroscopy

IVIS:

In vivo imaging system

RE:

Radiant efficiency

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Authors and Affiliations

  1. Department of Materials Science and Engineering, Graduate School of Science and Engineering, Ibaraki University, Hitachi, Japan

    Mayu Tayama, Tomoya Inose, Noriko Yamauchi, Kouichi Nakashima & Yoshio Kobayashi

  2. Department of Medical Physics, Graduate School of Medicine, Tohoku University, Sendai, Japan

    Tomoya Inose, Masayuki Tokunaga, Chihiro Kato & Kohsuke Gonda

  3. International Center for Synchrotron Radiation Innovation Smart (SRIS), Tohoku University, Sendai, Japan

    Kohsuke Gonda

Authors
  1. Mayu Tayama
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  2. Tomoya Inose
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  3. Noriko Yamauchi
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  4. Kouichi Nakashima
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  5. Masayuki Tokunaga
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  6. Chihiro Kato
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  7. Kohsuke Gonda
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  8. Yoshio Kobayashi
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Contributions

MT synthesized indocyanine green-loaded poly(lactic-co-glycolic acid) nanoparticles, investigated their morphology, and wrote the first draft. TI, MT, and CK performed the IVIS imaging. NY, KN, KG, and YK intensively discussed the results of investigation of particle morphology and IVIS imaging and completed the manuscript.

Corresponding author

Correspondence to Yoshio Kobayashi.

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Tayama, M., Inose, T., Yamauchi, N. et al. Fabrication and fluorescence imaging properties of indocyanine green-loaded poly(lactic-co-glycolic acid) nanoparticles. Colloid Polym Sci 299, 1271–1283 (2021). https://doi.org/10.1007/s00396-021-04844-3

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  • DOI: https://doi.org/10.1007/s00396-021-04844-3

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