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Identification of DNA markers associated with phenological traits in spring barley (Hordeum vulgare L.) under drought stress conditions

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Abstract

Drought is one of the important abiotic stresses that affects the phenological stages and crop productivity. Phenological stages can seriously influence the crop yield and quality. In this study, phenological traits including days to germination, days to tillering, days to stem elongation, days to head emergence, days to dough development in kernel, days to physiological maturity, number of grains per spike, thousand kernels weight, grain yield were evaluated in 148 commercial barley cultivars in both years under normal and drought stress conditions. In molecular experiment, the molecular profile of barley cultivars was assessed by 14 AFLP primer combinations and 32 SSRs loci. The phenotypic results showed the high level of diversity between studied cultivars. The studied barley cultivars were divided into two subgroups. Linkage disequilibrium analysis revealed that r2 values among all possible markers pairs have an average value of 0.0178. Mixed linear model procedure showed that totally, 137 loci had significant association with nine investigated traits under normal and drought stress conditions. The identified markers potentially can be used in marker-assisted selection in barley drought resistant breeding programs.

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Availability of data and materials

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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This research did not receive any specific grant from funding agencies in the public, commercial or not-for-profit sectors.

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Authors and Affiliations

  1. Faculty of Agriculture, Higher Educational Complex of Saravan, Saravan, Sistan and Baluchestan, Islamic Republic of Iran

    Mitra Jabbari

  2. Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Zabol, Zabol, Sistan and Baluchestan, Islamic Republic of Iran

    Barat Ali Fakheri, Nafiseh Mahdi Nezhad & Zahra Koochakpour

  3. Seed and Plant Improvement Research Department, Khorasan Razavi Agricultural and Natural Resources Research and Education Center, AREEO, Khorasan Razavi, Mashhad, Islamic Republic of Iran

    Reza Aghnoum

  4. Department of Plant Production and Genetics, Faculty of Agriculture and Natural Resources, Urmia University, Urmia, Islamic Republic of Iran

    Reza Darvishzadeh & Mitra Razi

  5. Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Alborz, Islamic Republic of Iran

    Reza Ataei

Authors
  1. Mitra Jabbari
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  2. Barat Ali Fakheri
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  3. Reza Aghnoum
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  5. Nafiseh Mahdi Nezhad
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  6. Reza Ataei
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  7. Zahra Koochakpour
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  8. Mitra Razi
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Contributions

Conceptualization, Methodology, Formal analysis and investigation: [Mitra Jabbari, Barat Ali Fakheri, Nafiseh Mahdi Nezhad, Reza Ataei, Zahra Koochakpour]; Resources: [Reza Aghnoum]; Writing—review and editing: [Reza Darvishzadeh, Mitra Razi].

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Correspondence to Reza Darvishzadeh.

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The authors declare that they have no competing interests.

Additional information

Communicated by A. Börner.

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Jabbari, M., Fakheri, B.A., Aghnoum, R. et al. Identification of DNA markers associated with phenological traits in spring barley (Hordeum vulgare L.) under drought stress conditions. CEREAL RESEARCH COMMUNICATIONS 50, 171–178 (2022). https://doi.org/10.1007/s42976-021-00181-x

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  • DOI: https://doi.org/10.1007/s42976-021-00181-x

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