STEP® vectors for rapid generation of stable transfected CHO cell pools and clones with high expression levels and product quality homogeneity of difficult-to-express proteins

https://doi.org/10.1016/j.pep.2021.105920Get rights and content
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Highlights

  • Difficult-to-express proteins expressed in CHO cells using STEP® vector technology.

  • STEP® technology enables production of DTE proteins with high product homogeneity.

  • STEP® vectors combine speed of transient expression with reliable stable expression.

  • Rapid production of any type of DTE protein for large-scale GMP manufacturing.

Abstract

Many proteins produced in CHO cells need evaluation for their clinical and commercial potential. Traditional methods based on stable clone generation are slow and unsuitable for screening larger numbers of proteins, while transient expression technologies are fast but unpredictable regarding product quality and lacking an optional path to subcloning. The STEP® vector technology introduced here combines the best properties of both methods. STEP® vectors contain a strong transcriptional cassette driving expression of a bicistronic mRNA. The gene-of-interest (GOI) is cloned upstream of a functionally impaired zeocin resistance gene (FI-Zeo) whose translation is coupled to that of the GOI through an IRES. Stable transfected cells surviving zeocin selection produce high levels of FI-Zeo and thus, high levels of the GOI-encoded protein. By using different spacers, the translational coupling efficiency and selection strength can be controlled allowing maximization of expression of any GOI. Production of laronidase and factor VII (FVII) is presented as examples of unrelated, difficult-to-express (DTE) proteins. First step is rapid generation of transfected pools with the STEP® vectors. All high expressing surviving pools showed high product quality homogeneity as did monoclonal cell lines obtained from the top pools. Up to 500 μg/mL laronidase was obtained with virtually identical glycosylation profile as reference product. For FVII, cell specific productivity of 0.45 pg/cell/day with 50 IU/μg protein matched highest reported levels of reference product even before process development. Taken together, STEP® vector technology is ideally suited for rapid, small to large-scale production of DTE proteins compared to traditional methods.

Keywords

Plasmid
Expression vector
Laronidase
CHO cell
Factor VII
Stable-transfected

Abbreviations

DTE
difficult-to-express
EE
expression enhancer
IRES
internal ribosomal entry site
GOI
gene of interest
FI-Zeo
functionally-impaired zeocin
ZeoR
zeocin resistance
IU
international units
USP
upstream process
FVII
factor VII
VCD
viable cell density
CV
coefficient of variance

Cited by (0)

1

Current affiliation: Pfizer

2

Current affiliation: Shanxi Biological Institute

3

Current affiliation: VectorY