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Genetic diversity of Amomum xanthioides and its related species from Southeast Asia and China

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Abstract

Amomum Semen, the seed mass of Amomum xanthioides, has been imported from Southeast Asia and China and used for the treatment of gastric and intestinal disorders. A. xanthioides has been treated as a synonym of A. villosum var. xanthioides. Furthermore, A. villosum var. villosum, A. villosum var. xanthioides, or A. longiligulare have been described as the botanical origin of Amomi Fructus, which is a similar crude drug in Chinese Pharmacopoeia. Under these circumstances, the botanical origin of Amomum Semen was changed to A. villosum var. xanthioides, A. villosum var. villosum, or A. longiligulare in Supplement II to the 17th edition of the Japanese Pharmacopoeia. To develop an objective identification method for Amomum Semen and to confirm the phylogenetic relationship among Amomum taxa, the nucleotide sequences of the nuclear ribosomal DNA internal transcribed spacer region and chloroplast DNA partial matK-trnK and trnH-psbA intergenic spacer regions were determined in specimens collected from Southeast Asia and China, including those from the type localities of each taxon. Six taxa were divided into four groups. A. xanthioides from Myanmar belonging to group 1 was discriminated from A. villosum var. xanthioides from China of group 2. A. villosum and its varieties were divided into two groups: group 2 included those from China, and group 3 consisted of A. villosum from Laos. A. longiligulare from China and Laos and A. uliginosum from Laos belonged to group 3 and group 4, respectively. These findings illustrate the phylogenetic basis for the need for taxonomical reorganization among the Amomum species.

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Acknowledgements

This work was supported in part by JSPS KAKENHI Grant Numbers 15H05268, and by the Research on Development of New Drugs from Japan Agency for Medical Research and Development, AMED [Grant number JP20ak0101046], as well as a research grant from Tsumura & Co.

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Authors and Affiliations

  1. Section of Pharmacognosy, Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama, 930-0194, Japan

    Mikako Sone, Shu Zhu & Katsuko Komatsu

  2. Kunming Institute of Botany, Chinese Academy of Science, Hoilongtan, Kunming, 650201, Yunnan, China

    Xiao Cheng

  3. Forestry Research Center, National Agriculture and Forestry Research Institute, Vientiane, Lao PDR

    Sounthone Ketphanh

  4. Department of Traditional Medicine, Ministry of Health and Sports, 47, Nay Pyi Taw, Myanmar

    Swe Swe & Than Lwin Tun

  5. Research Center for Medicinal Plant Resources, National Institutes of Biomedical Innovation, Health and Nutrition, 1-2 Hachimandai, Tsukuba, Ibaraki, 305-0843, Japan

    Noriaki Kawano & Nobuo Kawahara

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  1. Mikako Sone
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Correspondence to Katsuko Komatsu.

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Dr. Katsuko Komatsu received a research grant from Tsumura & Co.

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Sone, M., Zhu, S., Cheng, X. et al. Genetic diversity of Amomum xanthioides and its related species from Southeast Asia and China. J Nat Med 75, 798–812 (2021). https://doi.org/10.1007/s11418-021-01512-2

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