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Activity of Chemically Synthesized Peptide Encoded by the miR156A Precursor and Conserved in the Brassicaceae Family Plants

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Abstract

It was recently found that the primary transcripts of some microRNA genes (pri-miRNAs) are able to express peptides with 12 to 40 residues in length. These peptides, called miPEPs, participate in the transcriptional regulation of their own pri-miRNAs. In our previous studies, we used bioinformatic approach for comparative analysis of pri-miRNA sequences in plant genomes to identify a new group of miPEPs (miPEP-156a peptides) encoded by pri-miR156a in several dozen species of the Brassicaceae family. Exogenous miPEP-156a peptides could efficiently penetrate into the plant seedlings through the root system and spread systemically to the leaves. The peptides produced moderate morphological effect accelerating primary root growth. In parallel, the miPEP-156a peptides upregulated expression of their own pri-miR156a. Importantly, the observed effects at both morphological and molecular levels correlated with the peptide ability to quickly translocate into the cell nucleus and to bind chromatin. In this work, we established secondary structure of the miPEP-156a and demonstrated its changes induced by formation of the peptide complex with DNA.

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Notes

  1. * FAM fluorescence detection.

Abbreviations

CD:

circular dichroism

miRNA:

microRNA

miPEP:

peptide encoded by pri-miRNA

ORF:

open reading frame

pre-miRNA:

miRNA precursor

pri-miRNA:

miRNA gene primary transcript

qPCR:

quantitative polymerase chain reaction

TRAP:

translating ribosome affinity immunopurification

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Acknowledgments

The authors are grateful to A. A. Ignatova (Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry) for help with the CD experiments.

Funding

This work was financially supported by the Russian Foundation for Basic Research (project no. 19-04-00174-a).

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Authors and Affiliations

  1. Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia

    Tatiana N. Erokhina, Dmitry Yu. Ryazantsev, Larisa V. Samokhvalova, Andrey A. Mozhaev, Alexander N. Orsa & Sergey K. Zavriev

  2. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, 119992, Moscow, Russia

    Sergey Yu. Morozov

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  1. Tatiana N. Erokhina
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  2. Dmitry Yu. Ryazantsev
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  3. Larisa V. Samokhvalova
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  4. Andrey A. Mozhaev
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  5. Alexander N. Orsa
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  6. Sergey K. Zavriev
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  7. Sergey Yu. Morozov
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Correspondence to Sergey Yu. Morozov.

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Erokhina, T.N., Ryazantsev, D.Y., Samokhvalova, L.V. et al. Activity of Chemically Synthesized Peptide Encoded by the miR156A Precursor and Conserved in the Brassicaceae Family Plants. Biochemistry Moscow 86, 551–562 (2021). https://doi.org/10.1134/S0006297921050047

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