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Glabridin attenuates atopic dermatitis progression through downregulating the TLR4/MyD88/NF-κB signaling pathway

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Abstract

Background

Glabridin (GB), a bio-available phytoestrogen, displays various biological properties such as anti-inflammatory, antibacterial, and antiviral.

Objective

To explore the role of GB in the process of atopic dermatitis (AD).

Methods

CCK8 was used to detect the therapeutic effect of Glabridin in HaCat and NHEK cell inflammatory models. And evaluated the effect on cell proliferation and cell viability. The expression of TLR4, MyD88, P65 and P50 in HaCat and NHEK cell tissues was detected by qRT-PCR and PCR. At the same time, an AD animal model was constructed, and the cell experiment results were verified by hematoxylin–eosin (HE) and Immunohistochemistry staining (IHC).

Results

Enzyme-linked immunosorbent assay (ELISA) demonstrated that IL-1β, IL-6, and TNF-α upregulated by lipopolysaccharide (LPS) was decreased by treatment with GB. AD progression was further confirmed to be regulated by GB by inhibiting the TLR4/MyD88/NF-κB signaling pathway through real-time PCR and Western blot analyses. An AD-like mouse model demonstrated that GB considerably alleviated epidermal injury, relieve edema, and reduced inflammatory cell infiltration by H&E staining. Concurrently, IHC staining exhibited GB to reduce AD progression by impeding TLR4 expression.

Conclusion

GB was observed to decrease the AD progression by suppressing the TLR4/MyD88/NF-κB signaling pathway, which may likely serve as a novel therapeutic drug for AD management.

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Funding

The study was supported by the Scientific Research Fund of Inner Mongolia University for Nationalities (No. NMDYB1713).

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Authors and Affiliations

  1. Department of Dermatology, The Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao, China

    Jing Chang

  2. Department of Dermatological, DongguanTungwah Hospital, Dongguan, China

    Lin Wang

  3. Department of Traditional Chinese Medicine, The Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao, China

    Minna Zhang

  4. Rehabilitation Department, The Affiliated Hospital of Inner Mongolia University for Nationalities, Tongliao, China

    Zengjiao Lai

Authors
  1. Jing Chang
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  2. Lin Wang
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  3. Minna Zhang
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  4. Zengjiao Lai
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Contributions

JC conceived and designed the experiments. JC and LW performed the experiments and MZ, ZL analyzed the data. JC wrote the paper. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Jing Chang.

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Conflict of interest

Jing Chang, Lin Wang, Minna Zhang and Zengjiao Lai declare that there are no conflicts of interest.

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Chang, J., Wang, L., Zhang, M. et al. Glabridin attenuates atopic dermatitis progression through downregulating the TLR4/MyD88/NF-κB signaling pathway. Genes Genom 43, 847–855 (2021). https://doi.org/10.1007/s13258-021-01081-4

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  • DOI: https://doi.org/10.1007/s13258-021-01081-4

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