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Serum-derived exosomes accelerate scald wound healing in mice by optimizing cellular functions and promoting Akt phosphorylation

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Abstract

Wound exudate holds great clinical and research potential in wound repair via paracrine signaling. In essence, exudate is modified serum that contains a high concentration of exosomes. The aim of this study was to investigate the role of serum-derived exosomes in scald wound healing of NIH mice skin and to explore the underlying mechanisms. Hence, we constructed a deep second-degree scald model in NIH mice, testing the benefits of exosomes in the scald wound healing. The scratch wound assay, apoptosis assay and MTT assay were conducted to assess the effects of serum-derived exosomes on migration, apoptosis and proliferation of HaCaT cells and fibroblasts. Our results showed that serum-derived exosomes injected subcutaneously entered cells and effectively accelerated wound healing processes in mice. Additionally, serum-derived exosomes optimized functions of cells related to skin injury repair by stimulating fibroblast proliferation, promoting HaCaT cell migration, and suppressing apoptosis of HaCaT cells induced by heat stress. Further study revealed that serum-derived exosomes enhanced phosphorylation of the serine-threonine kinase Akt in scalded skin tissue. These results suggest a potential clinical use of serum-derived exosomes for treating skin scald.

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Acknowledgements

This work received funding from Guangzhou Science and Technology Project (201904010395) and The Guangdong Provincial Thousand-Hundred-Ten Talent Project (2015cxqx214).

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Author notes

  1. Yanwen Li and Yang Yu have contributed equally to this work.

Authors and Affiliations

  1. School of Biosciences and Biopharmaceutics, Guangdong Pharmaceutical University, Guangzhou, 510006, China

    Yanwen Li, Yang Yu, Zheng Xie, Xiaomin Ye, Xiaoyong Liu, Bin Xu & Jianwen Mao

  2. Guangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangzhou Higher Education Mega Center, Guangdong Pharmaceutical University, Guangzhou, 510006, Guangdong, People’s Republic of China

    Yanwen Li, Yang Yu, Zheng Xie, Xiaomin Ye, Xiaoyong Liu, Bin Xu & Jianwen Mao

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  1. Yanwen Li
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  2. Yang Yu
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Correspondence to Jianwen Mao.

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Li, Y., Yu, Y., Xie, Z. et al. Serum-derived exosomes accelerate scald wound healing in mice by optimizing cellular functions and promoting Akt phosphorylation. Biotechnol Lett 43, 1675–1684 (2021). https://doi.org/10.1007/s10529-021-03148-4

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  • DOI: https://doi.org/10.1007/s10529-021-03148-4

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