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APOBEC1 complementation factor facilitates cell migration by promoting nucleus translocation of SMAD3 in renal cell carcinoma cells

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Abstract

Metastasis is inevitable in about 30% of patients with primary renal cell carcinoma after nephrectomy treatment. APOBEC1 complementation factor (A1CF), an RNA binding protein, participates in tumor progressions such as growth, apoptosis, differentiation, and invasion. Here, we explored biological functions of A1CF and provided a new insight into renal cell carcinoma metastasis. Wound healing assay was conducted to detect migration in A1CF overexpression and knockdown stable cell lines. Quantitative PCR and western blot assays were utilized to test transcriptional and translation levels of A1CF and SMAD3 in A1CF overexpression and knockdown renal carcinoma cells. Nuclear and cytoplasmic protein separation assays were conducted to evaluate the subcellular distribution of A1CF and SMAD3. Immunoprecipitation assay was conducted to detect the interaction between A1CF and SMAD3. Our study demonstrated A1CF overexpression facilitated cell migration in renal carcinoma cells. A1CF deficiency downregulated expression of SMAD3, Snail1, and N-cadherin. In addition, A1CF promoted nucleus translocation of SMAD3 and interacted with SMAD3. SMAD3 knockdown attenuated cell migration induced by A1CF overexpression. Our study suggested A1CF facilitated cell migration by promoting nucleus translocation of SMAD3 in renal cell carcinoma cells.

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Acknowledgements

The authors are thankful for all the fellows in the M.O.E. Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University.

Funding

The work was funded by the National Science Foundation for Young Scientists of China (Grant No. 31701218) and the National Natural Science Foundation of China (Grant No. 82030065 and 81873932).

Author information

Authors and Affiliations

  1. The Ministry of Education Key Laboratory of Laboratory Medical Diagnostics, the College of Laboratory Medicine, Chongqing Medical University, Yuzhong District, Chongqing, 400016, China

    Hua Xia, Lei Xu, Huajie Mao, Qin Zhou & Yajun Xie

  2. State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, 650223, China

    Yamin Liu

Authors
  1. Hua Xia
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  2. Yamin Liu
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Correspondence to Yajun Xie.

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Additional information

Editor: Tetsuji Okamoto.

Supplementary Information

Supplementary Figure 1

A1CF upregulated SMAD3 expression in HEK293T cells. Protein levels and relative grayscale values of SMAD3 in A1CF overexpression (A) and knockdown (B, C) HEK293T cells. **P <0.01; ***P <0.001 (PNG 429 kb)

High resolution image (TIF 1152 kb)

Supplementary Figure 2

STR analyses of HEK293T cell line. Peak charts of locus in HEK293T cell line. (PNG 521 kb)

High resolution image (TIF 1166 kb)

Supplementary Figure 3

STR analyses of 786-O cell line. Peak charts of locus in 786-O cell line. (PNG 1247 kb)

High resolution image (TIF 2972 kb)

Supplementary Figure 4

STR analyses of A498 cell line. Peak charts of locus in A498 cell line. (PNG 1847 kb)

High resolution image (TIF 1925 kb)

Supplementary Figure 5

PCR analysis of mycoplasma infection. Agarose gel image of mycoplasma PCR detection. (PNG 348 kb)

High resolution image (TIF 986 kb)

Supplementary Table 1

The genotyping results of STR locus in HEK293T cell. STR profiles of HEK293T cell line were exactly matched with HEK293T (CVCL_QW54) in ExPASy database (8 core loci plus Amelogenin). (XLSX 10 kb)

Supplementary Table 2

The genotyping results of STR locus in 786-O cell. STR profiles of 786-O cell line were matched with 786-O (CVCL_1051) in ExPASy database. (XLSX 10 kb)

Supplementary Table 3

The genotyping results of STR locus in A498 cell. STR profiles of A498 cell line were matched with A498 (CVCL_1056) in ExPASy database. (XLSX 10 kb)

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Xia, H., Liu, Y., Xu, L. et al. APOBEC1 complementation factor facilitates cell migration by promoting nucleus translocation of SMAD3 in renal cell carcinoma cells. In Vitro Cell.Dev.Biol.-Animal 57, 501–509 (2021). https://doi.org/10.1007/s11626-021-00589-z

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  • DOI: https://doi.org/10.1007/s11626-021-00589-z

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