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24-epibrassinolide improves differential cadmium tolerance of mung bean roots, stems, and leaves via amending antioxidative systems similar to that of abscisic acid

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Abstract

Cadmium (Cd) pollution has attracted global concern. In the present study, the biochemical mechanisms underlying the amelioration of 24-epibrassinolide (eBL) and abscisic acid (ABA) on Cd tolerance of roots, stems, and leaves in mung bean seedlings were comparatively analyzed. Foliar application of eBL markedly ameliorated the growth of mung bean seedling exposed to 100 μM Cd. eBL alone had no significant effects on the activities of antioxidative enzymes and the contents of glutathione (GSH) and polyphenols in the three organs whereas significantly increased the root, stem, and leaf proline contents on average by 54.9%, 39.9%, and 94.4%, respectively, and leaf malondialdehyde (MDA) content on average by 69.0% compared with the controls. When the plants were exposed to Cd, eBL significantly reversed the Cd-increased root ascorbate peroxidase (APX) and superoxide dismutase (SOD) activities, root polyphenol, proline, and GSH levels, leaf chlorophyll contents, and MDA levels in the three organs. eBL significantly restored the Cd-decreased leaf catalase (CAT) activity and leaf polyphenol levels. These results indicated that eBL played roles in maintaining cellular redox homeostasis and evidently alleviated Cd-caused membrane lipid peroxidation via controlling the activity of antioxidative systems. eBL mediated the differential responses of cellular biochemical processes in the three organs to Cd exposure. Furthermore, a comparative analysis revealed that, under Cd stress, the effects of eBL on the biochemical processes were very similar to those of ABA, suggesting that ABA and eBL improve plant Cd tolerance via some common downstream pathways.

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This work was supported by the National Natural Science Foundation of China (31760110).

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  1. School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou, 730070, P. R. China

    Ya-Juan Song, Yi Li, Yan Leng & Shi-Weng Li

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  1. Ya-Juan Song
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LSW conceived of the study. SYJ, LY, and LY performed the experiments and data analyses. SYJ drafted the manuscript, and LSW reviewed the final manuscript. All authors read and approved the final manuscript.

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Song, YJ., Li, Y., Leng, Y. et al. 24-epibrassinolide improves differential cadmium tolerance of mung bean roots, stems, and leaves via amending antioxidative systems similar to that of abscisic acid. Environ Sci Pollut Res 28, 52032–52045 (2021). https://doi.org/10.1007/s11356-021-14404-5

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