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Podogigants A and B, two new potentiators of amphotericin B activity, from Sordariomycete Podostroma giganteum

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Abstract

Two new compounds, podogigants A (1) and B (2), were isolated from the culture broth of Podostroma giganteum. This is the first report on the identification of secondary metabolites in P. giganteum. The structures of 1 and 2 were elucidated through spectroscopic analysis, including 2D NMR spectroscopy assisted by chemical derivatization, which revealed the presence of farnesyl- and geranyl-hydroquinone structures, respectively. Compounds 1 and 2 exhibited no antifungal activity even at a concentration of 64 μg/mL, whereas they potentiated amphotericin B (AmB) activity against several species of fungi. In particular, 1 potentiated AmB activity against C. albicans and R. oryzae by up to 32-fold (MIC value of AmB decreased from 1.0 to 0.032 µg/mL), while 2 potentiated AmB activity against C. albicans by up to 16-fold.

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Acknowledgements

The analysis of podogigants A (1) and B (2) was carried out with AVANCE 500 and 600 at Chemical Analysis Division and the Open Facility, Research Facility Center for Science and Technology, University of Tsukuba. We would like to thank Editage (www.editage.com) for English editing. This work was partially supported by JSPS KAKENHI Grant Number JP20H05581.

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Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan

    Mikiko Hasegawa

  2. Department of Biology, National Museum of Nature and Science, 4-4-1 Amakubo, Tsukuba, Ibaraki, 305-0005, Japan

    Tsuyoshi Hosoya

  3. Graduate School of Pharmaceutical Science, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo, 108-8641, Japan

    Keisuke Kobayashi & Hiroshi Tomoda

  4. Faculty of Life and Environmental Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki, 305-8572, Japan

    Hideyuki Shigemori

  5. Microbiology Research Center for Sustainability (MiCS), University of Tsukuba, Tsukuba, Ibaraki, 305-8572, Japan

    Hideyuki Shigemori

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  1. Mikiko Hasegawa
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  2. Tsuyoshi Hosoya
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  3. Keisuke Kobayashi
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  4. Hiroshi Tomoda
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  5. Hideyuki Shigemori
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Correspondence to Hideyuki Shigemori.

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Hasegawa, M., Hosoya, T., Kobayashi, K. et al. Podogigants A and B, two new potentiators of amphotericin B activity, from Sordariomycete Podostroma giganteum. J Nat Med 75, 877–883 (2021). https://doi.org/10.1007/s11418-021-01531-z

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  • DOI: https://doi.org/10.1007/s11418-021-01531-z

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