Skip to main content
SpringerLink
Log in

Biomarkers of Systemic Inflammation in Local and Diffuse Peritonitis

  • Published:
Biochemistry (Moscow), Supplement Series B: Biomedical Chemistry Aims and scope Submit manuscript

Abstract

The progression of purulent inflammation in any acute surgical abdominal pathology can lead to local or diffuse peritonitis. The parameters characterizing the degree and specificity of the inflammatory response in blood such as cytokine concentration, neutrophil activity, plasma antioxidant capacity (thiols concentration) could be considered as potential predictors of complications. The luminol-dependent chemiluminescence (CL) response of blood activated by the phorbol ester (PMA), levels of cytokines IL-6, IL-8, IL-10, myeloperoxidase (MPO) and thiols were measured in plasma of patients with uncomplicated conditions (group 1, n = 8), local peritonitis (group 2, n = 9) or diffuse peritonitis (group 3, n = 9) at admission (before surgery, b/s), immediately after surgery (a/s) and a day after surgery (1 day) as well as in healthy volunteers (norm, n = 12). At all time-points the concentrations of cytokines and MPO measured by the ELISA method, in group 3 were higher than in healthy volunteers and in patients in groups 1 and 2. Blood CL demonstrated a more than 5-fold increase above the normal values in all patients, and it was also higher in group 2 as compared to group 1. In group 3 there were patients with both maximal and minimal CL values, which could be a consequence of neutrophil priming or exhaustion (“immune paralysis”), respectively. In these patients the plasma thiol content demonstrated a more than 3-fold decrease below normal values. In patients with fatal outcomes (group 3, n = 2) within a day after surgery, either a decrease of the CL to zero values concurrently with elevated IL-8 and IL-6 concentrations and low thiol levels was observed, or CL exceeded normal values more than 20 times with concurrent complete exhaustion of the plasma thiol pool. No clear dependency between the plasma parameters and neutrophil activity was found. This suggests that a panel of parameters for prognosis and/or early diagnostics of infectious complications in acute abdominal pathology should include different biomarkers of the inflammatory response: cytokine profile (IL-6, IL-8, IL-10), MPO and neutrophil activity, antioxidant plasma capacity (e.g., total thiols concentration).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.

Similar content being viewed by others

REFERENCES

  1. Tolonen, M., Kuuliala, K., Kuuliala, A., Leppänie-mi, A., Kulänpää, M.-L., Sallinen, V., Puolakka-inen, P., and Mentula, P., J. Surg. Res., 2019, vol. 244, pp. 436–443. https://doi.org/10.1016/j.jss.2019.06.081

    Article  CAS  PubMed  Google Scholar 

  2. Hong, C.W., Immune Netw., 2017, vol. 17, no. 5, pp. 298–306. https://doi.org/10.4110/in.2017.17.5.298

    Article  PubMed  PubMed Central  Google Scholar 

  3. Holzer, K., Konietzny, P., Wilhelm, K., and Henrich, D., Eur. Surg. Res., 2002, vol. 34, 275–284. https://doi.org/10.1159/000063071

    Article  CAS  PubMed  Google Scholar 

  4. Holzer, K., Richter, A., Hendrich, D., Wilhelm, K., and Encke, A., Shock, 2001, vol. 15, no. 2, pp. 90–94. https://doi.org/10.1097/00024382-200115020-00002

    Article  CAS  PubMed  Google Scholar 

  5. Guichard, C., Pedruzzi, E., Dewas, C., Fay, M., Pouzet, C., Bens, M., Vandewalle, E.O.D., Gougerot-Pocidalo, M.A., and Elbim, C., J. Biol. Chem., 2005, vol. 280, no. 44, pp. 37021–37032. https://doi.org/10.1074/jbc.M506594200

    Article  CAS  PubMed  Google Scholar 

  6. Quinlan, G.J., Margarson, M.P., Mumby, S., Evans, T.W., and Gutteridge, M.C., Clin. Sci., 1998, vol. 95, pp. 459–465.

    Article  CAS  Google Scholar 

  7. Kaysen, G.A., Dubin, J.A., Müller, H.-G., Rosales, L., Levin, N.W., and Mitch, W.E., Kidney International, 2004, vol. 65, no. 4, pp. 1408–1415. https://doi.org/10.1111/j.1523-1755.2004.00520.x

    Article  CAS  PubMed  Google Scholar 

  8. Vlasova, I.I., Mikhalchik, E.V., Gusev, A.A., Balabushevich, N.G., Gusev, S.A., and Kazarinov, K.D., Bioelectromagnetics, 2018, vol. 39, no. 2, pp. 144–155. https://doi.org/10.1002/bem.22103

    Article  CAS  PubMed  Google Scholar 

  9. Sokolov, A.V., Kostevich, V.A., Gorbunov, N.P., Grigorieva, D.V., Gorudko, I.V., Vasilyev, V.B., and Panasenko, O.M., Medical Immunology (Russia), 2018, vol. 20, no. 5, pp. 699–710. https://doi.org/10.15789/1563-0625-2018-5-699-710

    Article  Google Scholar 

  10. Sokolov, A.V., Kostevich, V.A., Zakharova, E.T., Samygina, V.R., Panasenko, O.M., and Vasilyev, V.B., Free Radical Research, 2015, vol. 46, no. 6, pp. 800–811. https://doi.org/10.3109/10715762.2015.1005615

    Article  CAS  Google Scholar 

  11. Hu, M.L., Methods Enzymol., 1994, vol. 233, pp. 380–385. https://doi.org/10.1016/s0076-6879(94)33044-1

    Article  CAS  PubMed  Google Scholar 

  12. Schrijver, I.T., Kemperman, H., Roest, M., Kesecioglu, J., and de Lange, D.W., Intensive Care Medicine Experimental, 2017, vol. 5, 43. https://doi.org/10.1186/s40635-017-0157-y

    Article  PubMed  PubMed Central  Google Scholar 

  13. Tanaka, T., Narazaki, M., and Kishimoto, T., Cold Spring Harb. Perspect. Biol., 2014, vol. 6, a016295, https://doi.org/10.1101/cshperspect.a016295

    Article  PubMed  PubMed Central  Google Scholar 

  14. Coopersmith, C.M., Amiot, II, D.M., Strom-berg, P.E., Dunne, W.M., Davis, C.G., Osborne, D.F., Husain, K.D., Turnbull, I.R., Karl, I.E., Hotch-kiss, R.S., and Buchman, T.G., Shock, 2003, vol. 19, no. 5, pp. 408–414. https://doi.org/10.1097/01.shk.0000054370.24363.ee

    Article  CAS  PubMed  Google Scholar 

  15. Ocuin, L.M., Bamboat, Z.M., Balachandran, V.P., Cavnar, M.J., Obaid, H., Plitas, G., and de Matteo, R.P., J. Leuk. Biol., 2011, vol. 89, pp. 423–432. https://doi.org/10.1189/jlb.0810479

    Article  CAS  Google Scholar 

  16. Zügel, N., Siebeck, M., Geißler, B., Lichtwark-Aschoff, M., Gippner-Steppert, C., Witte, J., and J-ochum, M., Arch. Surg., 2002, vol. 137, pp. 590–599.

    Article  Google Scholar 

  17. Craciun, F.L., Schuller, E.R., and Remick, D.G., J. Immunol., 2010, vol. 185, pp. 6930–6938. https://doi.org/10.4049/jimmunol.1002300

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Vogt, K.L., Summers, C., Chilvers, E.R., and Condliffe, A.M., Eur. J. Clin. Invest., 2018, vol. 48, suppl. 2, e12967. https://doi.org/10.1111/eci.12967

    Article  CAS  PubMed  Google Scholar 

  19. Wright, H.L., Cross, A.L., Edwards, S.W., and Moots, R.J., Rheumatology, 2014, vol. 53, pp. 1321–1331. https://doi.org/10.1093/rheumatology/keu035

    Article  CAS  PubMed  Google Scholar 

  20. Alves-Filho, J.C., Spiller, F., and Cunha, F.Q., Shock, 2010, vol. 34, suppl. 1, pp. 15–21. https://doi.org/10.1097/SHK.0b013e3181e7e61b

    Article  PubMed  Google Scholar 

  21. Taverna, M., Annals Intensive Care, 2013, vol. 3, 4. https://doi.org/10.1186/2110-5820-3-4

    Article  CAS  Google Scholar 

  22. Vlasova, I.I., Mikhalchik, E.V., Barinov, N.A., Kostevich, V.A., Smolina, N.V., Klinov, D.V., and Sokolov, A.V., Nanomedicine: Nanotechnology, Biology Medicine, 2016, vol. 12, pp. 1615–1625. https://doi.org/10.1016/j.nano.2016.02.012

    Article  CAS  Google Scholar 

Download references

Funding

This work was supported by the Russian Science Foundation (project no. 17-75-30064).

Author information

Authors and Affiliations

  1. Federal Research Clinical Center of Physical Chemical Medicine, ul. Malaya Pirogovskaya 1а, 119435, Moscow, Russia

    E. V. Mikhalchik, I. V. Borodina, T. V. Vakhrusheva, N. P. Gorbunov, O. M. Panasenko & D. V. Klinov

  2. Sechenov First Moscow State Medical University (Sechenov University), Institute for Regenerative Medicine, ul. Trubetskaya 8, bldg. 2, 119998, Moscow, Russia

    I. V. Vlasova

  3. Pirogov Russian National Research Medical University, ul. Ostrovityanova 1, 117997, Moscow, Russia

    S. M. Titkova, M. V. Anurov, G. B. Ivakhov & I. V. Ermakov

  4. Pirogov City Clinical Hospital no. 1, Leninskiy pr. 8, bldg.10, 119049, Moscow, Russia

    A. V. Teplyshev

Authors
  1. E. V. Mikhalchik
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. I. V. Borodina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. I. V. Vlasova
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. V. Vakhrusheva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. N. P. Gorbunov
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. O. M. Panasenko
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. S. M. Titkova
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. M. V. Anurov
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. G. B. Ivakhov
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. I. V. Ermakov
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. A. V. Teplyshev
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. D. V. Klinov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. V. Mikhalchik.

Ethics declarations

COMPLIANCE WITH ETHICAL STANDARDS

The study was performed in accordance with international ethical guidelines. Sampling and data processing were performed based on the informed consent of patients and healthy volunteers. The study was approved by the Local Ethics Committee of the Pirogov Russian National Research University (protocol no. 180 of December 17, 2018).

CONFLICT OF INTERESTS

The authors declare that they have no conflicts of interest.

SUPPLEMENTARY INFORMATION

Supplementary materials are available in the electronic version of the article at the journal’s website (pbmc.ibmc.msk.ru).

Additional information

Translated by A. Medvedev

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Mikhalchik, E.V., Borodina, I.V., Vlasova, I.V. et al. Biomarkers of Systemic Inflammation in Local and Diffuse Peritonitis. Biochem. Moscow Suppl. Ser. B 15, 125–131 (2021). https://doi.org/10.1134/S1990750821020074

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1990750821020074

Keywords:

Search

Navigation