Elsevier

Results in Chemistry

Volume 3, January 2021, 100134
Results in Chemistry

Steps toward enhancing the fluorescence of small-molecule-based protein labels using supramolecular hosts

https://doi.org/10.1016/j.rechem.2021.100134Get rights and content
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Abstract

Labeling of proteins with small-molecule-based fluorescent probes provides a powerful tool to determine their expression level and localization in living cells. We propose a method for increasing and stabilizing the emission of such probes using supramolecular hosts such as β-cyclodextrin (β-CD). Although the emission of a His-tag binding probe was enhanced in the presence of β-CD, binding of the probe-β-CD complex to a His-tagged protein led to partial β-CD displacement and consequently, a reduction in the emission signal.

Keywords

Fluorescent molecular probes
Cyclodextrin
Nile red
Supramolecular chemistry
Solvatochromic dyes

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