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The role of HIF-1α in BCG-stimulated macrophages polarization and their tumoricidal effects in vitro

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Abstract

BCG is widely used for cancer treatment, where macrophages play an important role. However, the mechanism of BCG affecting macrophages remains poorly understood. In this study, we used BCG to stimulate myeloid-derived macrophages lacking HIF-1α, the levels of TNF-α, IL-1β, CD86 of macrophages and their effects on the growth of tumor cells MCA207 and B16-F10 were detected. We found that the absence of HIF-1α prevents BCG-stimulated macrophages from polarizing towards the M (BCG) and attenuating its killing effect on tumor cells. In addition, we demonstrated that the tumors of mice lacking HIF-1α in macrophages were significantly increased by the experiment of mice transplantation. Our study provides relevant evidence for exploring the mechanism of the BCG vaccine in the prevention and treatment of related diseases.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Nos. 81871245) and Department of Education of Jilin Province (JJKH20190095KJ) and the Fundamental Research Funds for the Central Universities of China. The authors appreciate Dr. Zhinan Yin (The Biomedical Translational Research Institute, Jinan University, Guangzhou 510632 Guangdong, PR China) for providing the HIF-1α cKO mice.

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Authors and Affiliations

  1. Department of Immunology, College of Basic Medical Sciences, Jilin University, Changchun, China

    Pei Zhu, Yuyang Hou, Mengyan Tang, Zheng Jin, Dong Li & Dongmei Yan

  2. Shanghai University of Medicine and Health Sciences, Shanghai, China

    Youran Yu

  3. Department of critical care medicine, The Affiliated Hospital of Qingdao University, Qingdao, China

    Zehua Dong

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  1. Pei Zhu
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  2. Yuyang Hou
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Correspondence to Dongmei Yan or Zehua Dong.

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The animal study was reviewed and approved by Animal Research Committee of Jilin University.

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Zhu, P., Hou, Y., Tang, M. et al. The role of HIF-1α in BCG-stimulated macrophages polarization and their tumoricidal effects in vitro. Med Microbiol Immunol 210, 149–156 (2021). https://doi.org/10.1007/s00430-021-00708-3

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  • DOI: https://doi.org/10.1007/s00430-021-00708-3

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