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A common F-box gene regulates the leucine homeostasis of Medicago truncatula and Arabidopsis thaliana

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Abstract

The F-box domain is a conserved structural protein motif that most frequently interacts with the SKP1 protein, the core of the SCFs (SKP1-CULLIN-F-box protein ligase) E3 ubiquitin protein ligases. As part of the SCF complexes, the various F-box proteins recruit substrates for degradation through ubiquitination. In this study, we functionally characterized an F-box gene (MtF-box) identified earlier in a population of Tnt1 retrotransposon-tagged mutants of Medicago truncatula and its Arabidopsis thaliana homolog (AtF-box) using gain- and loss-of-function plants. We highlighted the importance of MtF-box in leaf development of M. truncatula. Protein–protein interaction analyses revealed the 2-isopropylmalate synthase (IPMS) protein as a common interactor partner of MtF-box and AtF-box, being a key enzyme in the biosynthesis pathway of the branched-chain amino acid leucine. For further detailed analysis, we focused on AtF-box and its role during the cell division cycle. Based on this work, we suggest a mechanism for the role of the studied F-box gene in regulation of leucine homeostasis, which is important for growth.

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All data generated or analyzed during this study are included in this manuscript [and its electronic supplementary material]. The data and the plant material will be available upon request.

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Acknowledgements

Part of the research was supported by EMBO fellowship (ASTF 506-2011) to Dr. Anelia Iantcheva, carried out at the Department of Plant Systems Biology, VIB, Ghent University, Ghent, Belgium, supervised by Prof. Lieven De Veylder. The authors wish to thank Dr. Valya Vassileva and Dr. Grigor Zehirov (Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences) for the establishment and maintenance of hydroponic culture of M. truncatula plants and technical help of Kety Krastanova (Agrobioinstitute).

Funding

Part of the research was supported by EMBO fellowship (ASTF 506–2011) to Dr. Anelia Iantcheva, carried out at the Department of Plant Systems Biology, VIB, Ghent University, Ghent, Belgium, supervised by Prof. Lieven De Veylder.

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Authors and Affiliations

  1. AgroBioInstitute, Agricultural Academy, Blvd. Dragan Tsankov 8, 1164, Sofia, Bulgaria

    Anelia Iantcheva, Miglena Revalska, Ivayla Dincheva & Ilian Badjakov

  2. Department of Plant Physiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, 8 Dragan Tsankov blvd., 1164, Sofia, Bulgaria

    Miroslava Zhiponova

  3. Department of Plant Biotechnology and Bioinformatics, Ghent University, B-9052, Ghent, Belgium

    Jefri Heyman, Nathan De Geyter, Sofie Goormachtig & Lieven De Veylder

  4. VIB Center for Plant Systems Biology, B-9052, Ghent, Belgium

    Jefri Heyman, Nathan De Geyter, Sofie Goormachtig & Lieven De Veylder

  5. Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Str., Bl. 21, 1113, Sofia, Bulgaria

    Irina Boycheva

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  1. Anelia Iantcheva
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Contributions

AI performed most of the experiments and data analyses. AI and MZ wrote the manuscript, designed and prepared the figures. MR performed phenotyping of A. thaliana transgenic lines, IB contributed with sample preparation for GC–MS of M. truncatula, JH performed F-box cell cycle localization and contributed for A. thaliana TAP analysis, IlB and ID performed the GC–MS analyses, NG was responsible for proteomics data of M. truncatula, SG was responsible for the experimental design of M. truncatula TAP data analyses, LV advised on experimental design and revised the paper. All the authors have read and approved the final manuscript.

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Correspondence to Anelia Iantcheva.

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Iantcheva, A., Zhiponova, M., Revalska, M. et al. A common F-box gene regulates the leucine homeostasis of Medicago truncatula and Arabidopsis thaliana. Protoplasma 259, 277–290 (2022). https://doi.org/10.1007/s00709-021-01662-w

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