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Enhanced Photodynamic Suppression of Enterococcus faecalis Using Curcumin-Loaded Zeolite

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Abstract

Antibacterial photodynamic therapy (aPDT) has drawn increasing attention as a noninvasive approach to remove bacterial contaminants such as E. faecalis from the tooth surface. In this study, curcumin (CUR) was loaded into ZSM-5 zeolite and the prepared photosensitizers (CUR@ZSM) were characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and ultraviolet–visible spectroscopy (UV–Vis). The loading efficiency, and release behavior of CUR were studied in CUR@ZSM. The antimicrobial and anti-biofilm potential of the photosensitizer were evaluated against E. faecalis via colony forming unit and crystal violet assays, respectively. No significant changes were observed in the size, morphology and crystallinity of the zeolite after CUR loading. CUR@ZSM showed a significant photodynamic inactivation effect through complete bacterial elimination and reduced the biofilm formation ability of E. faecalis up to about 80%. The results revealed that CUR@ZSM could be considered as a new potential photosensitizer for further study against endodontic infections.

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Acknowledgements

This work was supported by a grant from the Research Council of Tehran University of Medical Sciences (Grant Number: 42907).

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Author notes

  1. Aseyeh Ghaedi and Maryam Torshabi have the same contribution in this work.

Authors and Affiliations

  1. Department of Chemistry, Payame Noor University (PNU), P.O. Box, 19395-3697, Tehran, Iran

    Aseyeh Ghaedi, Afsaneh Rahimi Chahrogh & Ghasem Rezanejade Bardajee

  2. Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Science, Tehran, Iran

    Maryam Torshabi

  3. Department of Microbiology, Islamic Azad University, North Tehran Branch, Tehran, Iran

    Tayebeh Akbari

  4. Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran, Iran

    Sogol Saberi & Sima Shahabi

  5. Pharmaceutical Sciences and Cosmetic Products Research Center, Kerman University of Medical Sciences, Kerman, Iran

    Alieh Ameri

  6. Biomaterials Group, Pharmaceutical Sciences Research Center, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Tehran, Iran

    Mehdi Khoobi

  7. Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences, 14176, Tehran, Iran

    Mehdi Khoobi

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  1. Aseyeh Ghaedi
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Ghaedi, A., Torshabi, M., Chahrogh, A.R. et al. Enhanced Photodynamic Suppression of Enterococcus faecalis Using Curcumin-Loaded Zeolite. J Clust Sci 33, 1543–1551 (2022). https://doi.org/10.1007/s10876-021-02085-6

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