Abstract
Methionine is one of the many amino acids in the soil. In order to study the role of methionine in acidic forest soil, the effect of methionine (Met) was compared with control together with addition of ammonium (Met + A), nitrite (Met + N), and glucose (Met + C) under 60% or 90% water holding capacity (WHC), because ammonium and nitrite are important factors in nitrification, and glucose affect the heterotrophic nitrification and nitrogen immobilization. We found that methionine addition significantly reduced NO3− concentration in acidic forest soil. Compared to Met, Met + A and Met + N treatments non-significantly enhanced nitrification; however, Met + C treatment decreased NH4+ concentration which suggested that soil autotrophic and heterotrophic nitrification were limited in the presence of methionine at 60% WHC. Further, our findings of 15N-labeled treatment showed the impact and priming effect of methionine was negative for NO3− concentration and positive for N2O emission, which were observed mainly from the soil N source rather than methionine. At 90% WHC, Met + C treatment significantly lessened concentrations of NH4+ and NO3−, nonetheless improved N2O compared to Met treatment. Therefore, besides the denitrification and dissimilatory NO3− reduction to ammonia, the immobilization might be the key factor to explain this decrease in NO3− concentration at 90% WHC, while these processes were induced with the C addition. This study indicated that the positive role of amino acids in soil N cycling might be overrated.
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Some or all data that support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
We sincerely thank Yanyu Lin and Yuanzhen Peng for their assistance in soil samples analyses. The authors are very grateful to anonymous reviewers and responsible editors of this journal for valuable comments and suggestions to improve this manuscript.
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This research is supported by the National Natural Science Foundation of China (Grant Nos. 31770659, 31170578, 31570607, and 31470628).
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Ma, H., Tecimen, H.B., Pei, G. et al. Effects of co-addition of ammonium, nitrite, and glucose with methionine on soil nitrogen. Environ Monit Assess 193, 332 (2021). https://doi.org/10.1007/s10661-021-09109-8
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DOI: https://doi.org/10.1007/s10661-021-09109-8