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The snogI Gene is Necessary for the Proper Functioning of the Nogalamycin Biosynthesis Pathway

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A Correction to this article was published on 20 June 2021

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Abstract

Anthracycline drugs have multifunctional molecular structures, and small changes in the structure of the glycosyls around the chromophore affect their mechanism of action, pharmacokinetics, toxicity, anti-tumor activity, and many other significant parameters. DnrJ has a similar function to snogI but inverse stereoselectivity. SnogI encoding amino transferase was substituted for DnrJ for the purpose of obtaining nogalamycin analogues. We inactivated the snogI gene encoding an aminotransferase responsible for the formation of nogalamine and introduced the dnrJ gene encoding an aminotransferase responsible for the formation of daunosamine. We obtained the recombinant strain mLMX-3-100, in which the production of nogalamycin was disrupted. Interestingly, contrary to our predictions, no epi-nogalamycin was produced; nevertheless, the present study shows that the snogI gene is necessary for the proper functioning of the nogalamycin biosynthesis pathway. These data may provide a reference for further illustration of nogalamycin biosynthesis and its modification by way of combinatorial biosynthesis.biosynthesis and its modification by way of combinatorial biosynthesis.

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Acknowledgements

This research was supported by the Natural Science Foundation of Guangxi Province of China (2018GXNSFBA294004), Guangxi Autonomous Region University Student Innovation and Entrepreneurship Project (202010605054; 202010605068), Doctoral Foundation of Hubei University of Science and Technology Science (BK202028).

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Authors and Affiliations

  1. College of Clinical Medicine, HuBei University of Science and Technology, Xianning, China

    Mengxi Li

  2. Medicine Research Institute and Hubei Key Laboratory of Diabetes and Angiopathy, HuBei University of Science and Technology, Xianning, 437000, Hubei, China

    Zhenwang Zhang

  3. College of Chemical and Biological Engineering, Hechi University, Yizhou, 546300, Guangxi, China

    Mengxi Li

  4. Shanghai Institute of Pharmaceutical Industry, China National Institute of Pharmaceutical Industry, Shanghai, China

    Mengxi Li

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  1. Mengxi Li
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Correspondence to Zhenwang Zhang.

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The original online version of this article was revised: Modifications have been made to the Figures 3, 4B, 4C, 5 and 7. Full information regarding the corrections made can be found in the erratum/correction for this article.

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Li, M., Zhang, Z. The snogI Gene is Necessary for the Proper Functioning of the Nogalamycin Biosynthesis Pathway. Indian J Microbiol 61, 467–474 (2021). https://doi.org/10.1007/s12088-021-00941-7

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