Distribution and Community Composition of Anammox Bacteria in Shallow Groundwater of the Kathmandu Valley, Nepal

Mai Nakano; Tatsuru Kamei; Bijay Man Shakya; Takashi Nakamura; Yasuhiro Tanaka; Eiji Haramoto; Tadashi Toyama; Futaba Kazama

doi:10.1264/jsme2.ME20143

Short Communication

Distribution and Community Composition of Anammox Bacteria in Shallow Groundwater of the Kathmandu Valley, Nepal

Mai Nakano, Tatsuru Kamei, Bijay Man Shakya, Takashi Nakamura, Yasuhiro Tanaka, Eiji Haramoto, Tadashi Toyama, Futaba Kazama

Author information

Mai Nakano
Integrated Graduate School of Medicine, Engineering and Agricultural Science, University of Yamanashi
Tatsuru Kamei
Interdisciplinary Center for River Basin Environment, University of Yamanashi
Bijay Man Shakya
Integrated Graduate School of Medicine, Engineering and Agricultural Science, University of Yamanashi
Takashi Nakamura
Interdisciplinary Center for River Basin Environment, University of Yamanashi
Yasuhiro Tanaka
Department of Environmental Sciences, Faculty of Life and Environment Sciences, University of Yamanashi
Eiji Haramoto
Interdisciplinary Center for River Basin Environment, University of Yamanashi
Tadashi Toyama
Interdisciplinary Center for River Basin Environment, University of Yamanashi
Futaba Kazama
Interdisciplinary Center for River Basin Environment, University of Yamanashi

Keywords: anammox bacteria, distribution, groundwater, Kathmandu Valley

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Supplementary material

2021 Volume 36 Issue 1 Article ID: ME20143

DOI https://doi.org/10.1264/jsme2.ME20143

Details

Abstract

The abundance and diversity of anaerobic ammonium oxidation (anammox) bacteria were assessed in 152 groundwater samples in the Kathmandu Valley, Nepal. Anammox bacterial 16S rRNA genes were detected in 54% (37/68) of samples collected in the dry season at 1.6×10⁵–8.8×10⁶ copies L^–1, and in 60% (50/84) of samples collected in the wet season at 4.3×10⁴–1.2×10⁷ copies L^–1. The 16S rRNA genes of “Candidatus Brocadia”, “Candidatus Anammoxoglobus”, and five new deduced anammox bacterial phylotypes were detected in the shallow groundwater samples. Diverse anammox bacteria were broadly distributed in the shallow groundwater aquifer of the Kathmandu Valley.

Corresponding author

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