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Ginkgolide B derivative synthesis and their effects on the viability of SKOV3 cells

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Abstract

The natural product Ginkgolide B was used as a raw material and modified by esterification on C10-OH or C1-OH to obtain 11 derivatives (1–11), which were structurally characterized with nuclear magnetic resonance spectroscopy. An MTT assay-based in vitro tumor proliferation inhibitory activity test showed that compounds 2, 3, 6, 7, 10, and 11 exhibited strong inhibitory activity against the human ovarian cancer cells SKOV3, with IC50 values of 16.05 µmol/L, 15.65 µmol/L, 32.00 µmol/L, 63.30 µmol/L, 23.20 µmol/L, and 31.10 µmol/L, respectively. Annexin V/PI double staining assay showed that compound 2 induced apoptosis in SKOV3 cells to a slightly greater extent than GB and compounds 5 and 9, with an apoptosis rate of 31.68%.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (31702206), Key projects of science and Technology Department of Shaanxi Province (2018ZDXM-SF-083), and Natural Science Foundation of Shaanxi Province of China (2019JQ-530).

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Authors and Affiliations

  1. School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong, Shaanxi, China

    ZiLi Feng, ZhiBin Zhu, JiaYuan Xu & DaiHua Hu

  2. State Key Laboratory of Qinba Biological Resources and Ecological Environment, Hanzhong, Shaanxi, China

    ZiLi Feng, Wang Chen & Yu Bai

  3. Key Laboratory of Resource Biology of Shaanxi Province, Hanzhong, Shaanxi, China

    Wang Chen & Yu Bai

  4. Shaanxi Cuicheng Biomedical Technology Co., Ltd., Hanzhong, Shaanxi, China

    ZhengDong Zhao

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  1. ZiLi Feng
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Correspondence to Wang Chen.

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Feng, Z., Zhu, Z., Chen, W. et al. Ginkgolide B derivative synthesis and their effects on the viability of SKOV3 cells. Med Chem Res 30, 1265–1272 (2021). https://doi.org/10.1007/s00044-021-02732-7

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