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Structure of Microbial Complexes in Modelling of Polymetallic Pollution and Remediation of Agrosoddy-Podzolic Soils

  • ECOLOGY
  • Published:
Moscow University Soil Science Bulletin Aims and scope

Abstracts

In a model vegetation experiment (30 days), the responses of microbial communities of agro soddy-podzolic soil of two sites (Chashnikovo, Moscow oblast) with different organic carbon content (Corg 3.86 and 1.30%) to polymetallic pollution with heavy metals (HM) (Cu 660, Zn 1100, Pb 650 mg/kg) and treatment with biochar (5%) and lignohumate (0.25%). Classical microbiological culture-dependent analysis using Czapek agar and analysis of soil lipid profiles by gas chromatography–mass spectrometry revealed the differences in abundance colony forming units (CFUs), fungal and bacterial biomass, as well as in the diversity of cultivated fungi both in humus-rich and humus-poor soil. HMs did not have a significant effect on the number of CFUs and the number of cultural-morphological types of colonies, but reduced fungal and bacterial biomass in both soils; this decrease was more significant in a humus-poor soil. In addition, differences between the soils in the increase in the proportion of resistant melanized forms of fungi under the influence of HM in humus-rich (by 25.9%) and in humus-poor soil (by 45.7%) were observed. The sensitivity and universal significance of structural indicators as indicators of the stability of microbial complexes under chemical contamination of soils with different humus content were discussed. It was shown that the most sensitive and reliable indicators among the studied included the proportion of melanized fungi.

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ACKNOWLEDGMENTS

Authors are grateful to P.V. Uchanov and E.V. Prudnikova for assistance in the collection of soil samples, M.M. Karpukhin for the chemical analysis of soils, Research and Production Association “Realization of Environmental Technologies” (NPO RET LLC) for the provided samples of lignohumate, and Metacom LLC for cooperation in assessing the effects of biochar.

Funding

The work was supported by RFBR, project no. 18-04-01218a “Study of the distribution of the occurrence of micromycete species to assess the ecological risk of contaminated soils before and after remediation”. This research was performed according to the Development program of the Interdisciplinary Scientific and Educational School of Moscow State University “The future of the planet and global environmental change.”

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Authors and Affiliations

  1. Department of Soil Science, Moscow State University, Moscow, Russia

    V. A. Terekhova, Ju. V. Belfeg, A. G. Rychagova & N. V. Verkhovtseva

  2. Pirogov Russian National Research Medical University, Moscow, Russia

    E. V. Fedoseeva

  3. Severtsov Institute of Ecology and Evolution of the Russian Academy of Sciences, Moscow, Russia

    V. A. Terekhova & A. P. Kiryushina

Authors
  1. V. A. Terekhova
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  2. E. V. Fedoseeva
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  3. Ju. V. Belfeg
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  4. A. P. Kiryushina
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  5. A. G. Rychagova
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  6. N. V. Verkhovtseva
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Correspondence to V. A. Terekhova.

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Conflict of interests. The authors declare that they have no conflicts of interest.Statement on the welfare of humans or animals. This article does not contain any studies involving animals performed by any of the authors.

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Translated by A. Bulaev

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Terekhova, V.A., Fedoseeva, E.V., Belfeg, J.V. et al. Structure of Microbial Complexes in Modelling of Polymetallic Pollution and Remediation of Agrosoddy-Podzolic Soils. Moscow Univ. Soil Sci. Bull. 76, 33–40 (2021). https://doi.org/10.3103/S0147687421010051

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