Skip to main content

Advertisement

SpringerLink
Log in

Supplementation of fly ash improves growth, yield, biochemical, and enzymatic antioxidant response of chickpea (Cicer arietinum L.)

  • Research Report
  • Published:
Horticulture, Environment, and Biotechnology Aims and scope Submit manuscript

Abstract

This research was investigated to assess the effect of fly ash (FA) on the growth, yield, biochemical attributes, and enzymatic antioxidant response of chickpea (Cicer arietinum L. cv. Avrodhi). After evaluating soil and FA nutrient status by energy-dispersive X-ray spectroscopy (EDX), different concentrations of FA with soil were applied (5, 10, 15, 20, 25, and 30%). Chickpea plants grown at all FA-amended soil concentrations demonstrate significant improvement (p ≤ 0.05) in growth and yield biomass. Exhibition of enhancement in biochemical attributes such as photosynthetic pigments (chlorophyll a and b, total chlorophyll, and total carotenoid by 16.83, 24.50, 19.13, and 21.74%), total protein (48.54%), leghemoglobin (42.39%), nitrate reductase activity (18.35%), proline content (28.16%), and antioxidant enzymes activities. Moreover, an improvement in the stomatal pore of the FA treated plant also noticed by a scanning electron microscope (SEM). Overall, the findings of this study indicate that the supplementation of FA contributed to increment in these attributes at all the concentrations compared with control (without FA) ‘but maximally ~ up to FA 20%.’ However, plant growth and yield biomass decreased above the concentration of FA 20% because chickpea can balanced nutrients efficiency up to FA 20%.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  • Bates LS, Walden RT, Tearse ID (1973) Rapid determination of free proline for water stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Beauchamp LO, Fridovich I (1971) Superoxide dismutase improved assays and assay applicable to acrylamide gels. Ann Biochem 44:276–287

    Article  CAS  Google Scholar 

  • Bisoi SS, Mishra SS, Barik J, Panda D (2017) Effects of different treatments of fly ash and mining soil on growth and antioxidant protection of Indian wild rice. Int J Phytoremediation 19:446–452

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Cakmak I (2005) The role of potassium in alleviating detrimental effects of abiotic stresses in plants. J Plant Nutr Soil Sci 168:521–530

    Article  CAS  Google Scholar 

  • CEA (2017–2018) Report on fly ash generation at coal/lignite based thermal power stations and its utilization in the country for the year 2017–2018. Central Electricity Authority, New Delhi, India

  • Chance B, Maehly AC (1955) Assay of catalase and peroxidase. Methods Enzymol 2:764–775

    Article  Google Scholar 

  • FAOSTAT (2019) Chickpea production in 2018, crops/regions/word list/production quantity (pick lists). http://www.fao.org/faostat/en/#data/QC. Accessed 28 Apr 2020

  • Hadke PB, Wankhade SG, Ingle SN (2019) Consequence of fym and fly ash application on yield, nutrient uptake, and quality of safed musli grown on inceptisols. Int J Curr Microbiol Appl Sci 8:263–273

    Article  CAS  Google Scholar 

  • Haris M, Ahmad G, Shakeel A, Khan AA (2019) Utilization of fly Ash to improve the growth and the management of root-knot nematode on carrot. Haya Saudi J Life Sci 4:221–226

    Google Scholar 

  • Hell R, Hillebrand H (2001) Plant concepts for mineral acquisition and allocation. Curr Opin Biotechnol 12:161–168

    Article  CAS  Google Scholar 

  • Jaworski EG (1971) Nitrate reductase assay in intact plant tissues. Biochem Biophys Res Commun 43:1274–1279

    Article  CAS  Google Scholar 

  • Jukanti AK, Gaur PM, Gowda CLL, Chibbar RN (2012) Nutritional quality and health benefits of chickpea (Cicer arietinum L.): a review. Br J Nutr 108:11–26

    Article  Google Scholar 

  • Maclachlan S, Zalik S (1963) Plastid structure, chlorophyll concentration, and free amino acid composition of chlorophyll mutant barley. Can J Bot 41:1053–1062

    Article  CAS  Google Scholar 

  • Marschner P (2012) Mineral nutrition of higher plants, 3rd edn. Academic Press, London

    Google Scholar 

  • Moorby J, Besford RT (1983) Mineral nutrition and growth. In: Lauchli A, Bieleski RL (eds) Encyclopedia of plant physiology, vol 15B. Springer, Berlin, pp 81–527

    Google Scholar 

  • Ochecova P, Tlustos P, Szakova J (2014) Wheat and soil response to wood fly ash application in contaminated soils. Agron J 206:995–1002

    Article  Google Scholar 

  • Panda D, Panda D, Padhan B, Biswas M (2018) Growth and physiological response of lemongrass (Cymbopogon citratus (DC) Stapf.) under different levels of fly ash-amended soil. Int J Phytoremediation 20:538–544

    Article  CAS  Google Scholar 

  • Panda RB, Biswal T (2018) Impact of fly ash on soil properties and productivity. Int J Agric Environ Biotechnol 11:275–283

    Google Scholar 

  • Pandey VC, Abhilash PC, Upadhyay RN, Tewari DD (2009) Application of fly ash on the growth performance and translocation of toxic heavy metals within Cajanus cajan L: implication for safe utilization of fly ash for agricultural production. J Hazard Mater 166:255–259

    Article  CAS  Google Scholar 

  • Pandey VC, Singh N (2010) Impact of fly ash incorporation in soil systems. Agric Eco Environ 136:16–27

    Article  Google Scholar 

  • Qurratul JS, Khan R, Mahmooduzzafar STO (2013) Soil amendments of fly ash: effects on function and biochemical activity of Carthamus tinctorius L. plants. Isr J Plant Sci 61:12–24

    Article  Google Scholar 

  • Sadasivam S, Manickam A (1992) Chlorophylls. Biochemical Methods for Agricultural Sciences. Wiley Eastern Limited, New Delhi, pp 184–185

    Google Scholar 

  • Saito K (2000) Regulation of sulfate transport and synthesis of sulfur containing amino acids. Curr Opin Plant Biol 3:188–195

    Article  CAS  Google Scholar 

  • Shakeel A, Khan AA, Hakeem KR (2020) Growth, biochemical, and antioxidant response of beetroot (Beta vulgaris L.) grown in fly ash-amended soil. SN Appl Sci 2:1378

    Article  CAS  Google Scholar 

  • Shamsi IH, Jilani G, Guo-Ping Z, Kang W (2008) Cadmium stress tolerance through potassium nutrition in soybean. Asian J Chem 20:1099–1108

    CAS  Google Scholar 

  • Sharma B, Singh RP (2019) Physiological, biochemical, growth, and yield responses of Radish (Raphanus sativus L.) plants grown on different sewage sludge-fly ash mixture (SLASH) ratios. In: Ghosh S (ed) Waste valorisation and recycling. Springer, Singapore, pp 539–552

    Chapter  Google Scholar 

  • Siddique KH, Johansen C, Turner NC, Jeuffroy MH, Hashem A, Sakar D, Gan Y, Alghamdi SS (2012) Innovations in agronomy for food legumes. A review. Agron Sustain Dev 32:45–64

    Article  Google Scholar 

  • Skousen J, Yang JE, Lee JS, Ziemkiewicz P (2013) Review of fly ash as a soil amendment. Geosys Eng 16:249–256

    Article  CAS  Google Scholar 

  • Thind HS, Singh Y, Singh B, Singh V, Sharma S, Vashistha M, Sing G (2012) Land application of rice husk ash, bagasse ash and coal fly ash: effects on crop productivity and nutrient uptake in rice-wheat system on an alkaline loamy sand. Field Crop Res 135:137–144

    Article  Google Scholar 

  • Ukwattage NL, Ranjith PG, Bouazza M (2013) The use of coal combustion fly ash as a soil amendment in agricultural lands (with comments on its potential to improve food security and sequester carbon). Fuel 109:400–408

    Article  CAS  Google Scholar 

  • Verma SK, Singh K, Gupta AK, Pandey VC, Trivedi P, Verma RK, Patra DD (2014) Aromatic grasses for phytomanagement of coal fly ash hazards. Ecol Eng 73:425–428

    Article  Google Scholar 

  • Wang YL, Wei MY, Chen SJ (2008) Effects of Cr6+ on the growth and physiological and biochemical characteristics of Mentha crispata. J Anhui Agric Sci 36:7100–7102

    CAS  Google Scholar 

  • Wong JWC, Wong MH (1990) Effects of fly ash on yields and elemental composition of two vegetables, Brassica parachinensis and B. chinensis. Agric Ecosyst Environ 30:251–264

    Article  CAS  Google Scholar 

  • Yamasaki S, Dillenburg LC (1999) Measurements of leaf relative water content in Araucaria angustifolia. Rev Bras Fisiol Veg 11:69–75

    Google Scholar 

Download references

Acknowledgements

The authors wish to thank Department of Botany, AMU to carry out the research work. We would like to extend our gratitude to University Sophisticated Instrumentation Facility (USIF), Aligarh Muslim University (AMU) for providing the SEM and EDX facilities.

Author information

Authors and Affiliations

  1. Section of Plant Pathology and Environmental Botany, Department of Botany, Aligarh Muslim University, Aligarh, U.P., 202002, India

    Mohammad Haris, Moh Sajid Ansari & Abrar Ahmad Khan

Authors
  1. Mohammad Haris
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Moh Sajid Ansari
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Abrar Ahmad Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

MH and AAK designed the experimental work. MH and MSA performed the experimental work. MH has done the statistical analysis, and formatting of the manuscript. AAK has done the final editing of the manuscript. The manuscript has been read and approved by all the authors.

Corresponding author

Correspondence to Mohammad Haris.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Communicated by Sung Kyeom Kim.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Haris, M., Ansari, M.S. & Khan, A.A. Supplementation of fly ash improves growth, yield, biochemical, and enzymatic antioxidant response of chickpea (Cicer arietinum L.). Hortic. Environ. Biotechnol. 62, 715–724 (2021). https://doi.org/10.1007/s13580-021-00351-0

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13580-021-00351-0

Keywords

Search

Navigation