Abstract
Microbially induced calcite precipitation (MICP) technique has gained attention recently as a novel method to enhance the engineering properties of soils, especially sandy soils. However, the applicability of this method to field scale is challenging and requires understanding of the factors affecting MICP process under variable subsurface conditions. This study presents a laboratory investigation and numerical predictive model to assess pore-water chemistry and calcite precipitation during the biocementation process. Laboratory experiments were conducted to assess the microbial treatment of Narmada sand in plastic tubes using three bacterial strains and two cementation media concentrations. Calcite precipitation via ureolysis as a result of biogeochemical reactions was measured. The effects of pH and electrical conductivity (EC) on the rate of urea hydrolysis and calcite precipitation were assessed. The presence of calcite crystals was analyzed using scanning electron microscopy (SEM). The SEM images confirmed the formation of calcite at the surface and between the sand particles. A simplified numerical model was developed to estimate the rate of urea hydrolysis and their effects on the biocementation of sand. Three stages of MICP process were identified: bacterial ureolysis, dynamic equilibrium between liquid-gas interface and oversaturation of ions, and calcite precipitation. The variations of pH and EC at these three stages were modeled. The predicted pH, EC, and calcite precipitation based on the simplified model were found to be in close agreement with the experimental results. The numerical model can be used to assess and optimize the system variables for effective MICP field applications.
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References
Al Qabany A, Soga K (2013) Effect of chemical treatment used in MICP on engineering properties of cemented soils. Geotechnique 63:331–339. 10.1680/geot.SIP13.P.022
Al Qabany A, Soga K, Santamarina C (2012) Factors affecting efficiency of microbially induced calcite precipitation. J Geotech Geoenviron Eng 138:992–1001. https://doi.org/10.1061/(asce)gt.1943-5606.0000666
Amin M, Zomorodian SMA, O’Kelly BC (2017) Reducing the hydraulic erosion of sand using microbial-induced carbonate precipitation. Proc Inst Civ Eng - Gr Improv 170:112–122. https://doi.org/10.1680/jgrim.16.00028
Anbu P, Kang CH, Shin YJ, So JS (2016) Formations of calcium carbonate minerals by bacteria and its multiple applications. Springerplus 5:1–26
Barkouki T, Martinez B, Mortensen B (2009) Forward and inverse bio-geochemical modeling of microbially induced precipitation in 0.5 M columnar experiments. TOUGH Symp 2009:1–8
Batstone DJ, Keller J, Angelidaki I et al (2002) Industrial applications of the IWA anaerobic digestion model No 1 (ADM1). Water Sci Technol 45:65–73
Bu C, Wen K, Liu S et al (2018) Development of bio-cemented constructional materials through microbial induced calcite precipitation. Mater Struct Constr 51:1–11. https://doi.org/10.1617/s11527-018-1157-4
Chu J, Ivanov V, Stabnikov V, Li B (2013) Microbial method for construction of an aquaculture pond in sand. Geotechnique 63:871–875. https://doi.org/10.1680/geot.SIP13.P.007
Clark MP, Nijssen B, Lundquist JD, et al. (2015) A revised model for microbially induced calcite precipitation: improvements and new insights based on recent experiments. Water Resour Res 2498–2514. https://doi.org/10.1002/2015WR017200.A
Covington AK, Bates RG, Durst RA (1985) Definition of pH scales, standard reference values, measurement of pH and related terminology. Pure Appl Chem 57:531–542. https://doi.org/10.1351/pac198557030531
Cui MJ, Zheng JJ, Chu J, et al. (2020) Bio-mediated calcium carbonate precipitation and its effect on the shear behaviour of calcareous sand. Acta Geotech: https://doi.org/10.1007/s11440-020-01099-0
Cuthbert MO, McMillan LA, Handley-Sidhu S et al (2013) A field and modeling study of fractured rock permeability reduction using microbially induced calcite precipitation. Environ Sci Technol 47:13637–13643. https://doi.org/10.1021/es402601g
De Muynck W, Verbeken K, De Belie N, Verstraete W (2010) Influence of urea and calcium dosage on the effectiveness of bacterially induced carbonate precipitation on limestone. Ecol Eng 36:99–111. https://doi.org/10.1016/j.ecoleng.2009.03.025
DeJong JT, Fritzges MB, Nüsslein K (2006) Microbially induced cementation to control sand response to undrained shear. J Geotech Geoenviron Eng 132:1381–1392. https://doi.org/10.1061/(asce)1090-0241(2006)132:11(1381)
Dupraz S, Parmentier M, Ménez B, Guyot F (2009) Experimental and numerical modeling of bacterially induced pH increase and calcite precipitation in saline aquifers. Chem Geol 265:44–53. https://doi.org/10.1016/j.chemgeo.2009.05.003
Estiu G, Merz KM (2006) Catalyzed decomposition of urea. Molecular dynamics simulations of the binding of urea to urease. Biochemistry 45:4429–4443. https://doi.org/10.1021/bi052020p
Ferris FG, Stehmeier LG, Kantzas A, Mourits FM (1996) Bacteriogenic mineral plugging. J Can Pet Technol 35:56–61. https://doi.org/10.2118/96-08-06
Ferris FG, Phoenix V, Fujita Y, Smith RW (2003) Kinetics of calcite precipitation induced by ureolytic bacteria at 10 to 20 °C in artificial groundwater. Geochim Cosmochim Acta 67:1701–1722. https://doi.org/10.1016/S0016-7037(03)00503-9
Gat D, Tsesarsky M, Shamir D, Ronen Z (2014) Accelerated microbial-induced CaCO3 precipitation in a defined coculture of ureolytic and non-ureolytic bacteria. Biogeosciences 11:2561–2569. https://doi.org/10.5194/bg-11-2561-2014
Hales JM, Drewes DR (1979) Solubility of ammonia in water at low concentrations. Atmos Environ 13:1133–1147. https://doi.org/10.1016/0004-6981(79)90037-4
Helmi FM, Elmitwalli HR, Elnagdy SM, El-Hagrassy AF (2016) Calcium carbonate precipitation induced by ureolytic bacteria Bacillus licheniformis. Ecol Eng 90:367–371. https://doi.org/10.1016/j.ecoleng.2016.01.044
International Association for the Properties of Water and Steam (2007) Release on the ionization constant of H2O
IS: 2720 (Part 14) (1983) Indian standard: methods of test for soils, part 14: determination of density index (relative density) of cohesionless soils. Bur Indian Stand New Delhi 1–15
IS: 2720 (Part 17) (1986) Indian standard: methods of test for soils, part 17: laboratory determination of permeability. Bur Indian Stand New Delhi 1–14
IS: 2720 (Part 3/Sec 1) (1980) Indian standard: methods of test for soils, part 3: determination of specific gravity, section 1: fine grained soils. Bur Indian Stand New Delhi 1–9. 10.1093/jaoac/20.3.535
IS: 2720 (Part 4) (1985) Indian standard: methods of test for soils, part 4: grain size analysis. Bur Indian Stand New Delhi 1–39
IS: 2720 (Part 7) (1980) Indian standard: methods of test for soils, part 7: determination of water content-dry density relation using light compaction. Bur Indian Stand New Delhi 1–10
IS:1498 (1970) Indian standard: classification and identification of soils for general engineering purposes. Bur Indian Stand New Delhi 1–27
Ivanov V, Chu J (2008) Applications of microorganisms to geotechnical engineering for bioclogging and biocementation of soil in situ. Rev Environ Sci Biotechnol 7:139–153
Keykha HA, Asadi A, Zareian M (2017) Environmental factors affecting the compressive strength of microbiologically induced calcite precipitation treated soil. Geomicrobiol J 34:889–894. https://doi.org/10.1080/01490451.2017.1291772
Lei X, Lin S, Meng Q et al (2020) Influence of different fiber types on properties of biocemented calcareous sand. Arab J Geosci 13:317. https://doi.org/10.1007/s12517-020-05309-7
Liu P, Shao G h, Huang R p (2019) Study of the interactions between S. pasteurii and indigenous bacteria and the effect of these interactions on the MICP. Arab J Geosci 12:724. https://doi.org/10.1007/s12517-019-4840-z
Madigan MT, Martinko JM (2003) Brock biology of microorganisms, 11th edn. Pearson Education Inc, Upper Saddle River, NJ, USA
Mahawish A, Bouazza A, Gates WP (2019) Factors affecting the bio-cementing process of coarse sand. In: Proceedings of the Institution of Civil Engineers: Ground Improvement. ICE Publishing, pp 25–36
Millo C, Dupraz S, Ader M et al (2012) Carbon isotope fractionation during calcium carbonate precipitation induced by ureolytic bacteria. Geochim Cosmochim Acta 98:107–124. https://doi.org/10.1016/j.gca.2012.08.029
Minto JM, Lunn RJ, El Mountassir G (2019) Development of a reactive transport model for field-scale simulation of microbially induced carbonate precipitation. Water Resour Res 55:7229–7245. https://doi.org/10.1029/2019WR025153
Mitchell AC, Ferris FG (2005) The coprecipitation of Sr into calcite precipitates induced by bacterial ureolysis in artificial groundwater: temperature and kinetic dependence. Geochim Cosmochim Acta 69:4199–4210. https://doi.org/10.1016/j.gca.2005.03.014
Mitchell JK, Santamarina JC (2005) Biological considerations in geotechnical engineering. J Geotech Geoenviron Eng 131:1222–1233. https://doi.org/10.1061/(asce)1090-0241(2005)131:10(1222)
Mitchell AC, Espinosa-Ortiz EJ, Parks SL et al (2019) Kinetics of calcite precipitation by ureolytic bacteria under aerobic and anaerobic conditions. Biogeosciences 16:2147–2161. https://doi.org/10.5194/bg-16-2147-2019
Mohammadizadeh M, Ajalloeian R, Nadi B, Nezhad SS (2020) Experimental study on soil improvement using local microorganisms. Arab J Geosci 13:469. https://doi.org/10.1007/s12517-020-05450-3
Montoya BM, Dejong JT (2015) Stress-strain behavior of sands cemented by microbially induced calcite precipitation. J Geotech Geoenviron Eng 141:04015019. https://doi.org/10.1061/(ASCE)GT.1943-5606
Moravej S, Habibagahi G, Nikooee E, Niazi A (2018) Stabilization of dispersive soils by means of biological calcite precipitation. Geoderma 315:130–137. https://doi.org/10.1016/j.geoderma.2017.11.037
Mujah D, Shahin MA, Cheng L (2017) State-of-the-art review of biocementation by microbially induced calcite precipitation (MICP) for soil stabilization. Geomicrobiol J 34:01–15. https://doi.org/10.1080/01490451.2016.1225866
O’Donnell ST, Kavazanjian E (2015) Stiffness and dilatancy improvements in uncemented sands treated through MICP. J Geotech Geoenviron Eng 141:02815004. https://doi.org/10.1061/(ASCE)GT.1943-5606.0001407
Okwadha GDO, Li J (2010) Optimum conditions for microbial carbonate precipitation. Chemosphere 81:1143–1148. https://doi.org/10.1016/j.chemosphere.2010.09.066
Qian CX, Rong H, Yu XN, Wang X (2016) Experiments on and predictions about properties of sand bonded by microbe cement. Sci China Technol Sci 59:1186–1193. https://doi.org/10.1007/s11431-016-6077-3
Sharma M, Satyam N, Reddy KR (2019) Investigation of various gram-positive bacteria for MICP in Narmada Sand, India. Int J Geotech Eng 1–15. https://doi.org/10.1080/19386362.2019.1691322
Sharma M, Satyam N, Reddy KR (2020) Strength enhancement and lead immobilization of sand using consortia of bacteria and blue-green algae. J Hazardous, Toxic, Radioact Waste 24:04020049. https://doi.org/10.1061/(ASCE)HZ.2153-5515.0000548
Sharma M, Satyam N, Reddy KR (2021a) Hybrid bacteria mediated cemented sand: microcharacterization, permeability, strength, shear wave velocity, stress-strain, and durability. Int J Damage Mech 1–28. https://doi.org/10.1177/1056789521991196
Sharma M, Satyam N, Reddy KR (2021b) Effect of freeze-thaw cycles on engineering properties of biocemented sand under different treatment conditions. Eng Geol 284:106022. https://doi.org/10.1016/j.enggeo.2021.106022
Sharma M, Satyam N, Reddy KR (2021c) State of the art review of emerging and biogeotechnical methods for liquefaction mitigation in sands. J Hazardous, Toxic, Radioact Waste 25:03120002. https://doi.org/10.1061/(asce)hz.2153-5515.0000557
Sharma M, Satyam N, Reddy KR (2021d) Comparison of improved shear strength of biotreated sand using different ureolytic strains and sterile conditions. Soil Use Manag. https://doi.org/10.1111/sum.12690
Sharma M, Satyam N, Reddy KR (2021e) Rock-like behavior of biocemented sand treated under non-sterile environment and various treatment conditions. J Rock Mech Geotech Eng. https://doi.org/10.1016/j.jrmge.2020.11.006
Stocks-Fischer S, Galinat JK, Bang SS (1999) Microbiological precipitation of CaCO3. Soil Biol Biochem 31:1563–1571. https://doi.org/10.1016/S0038-0717(99)00082-6
Tiwari N, Satyam N (2019) Experimental study on the influence of polypropylene fiber on the swelling pressure expansion attributes of silica fume stabilized clayey soil. Geosciences 9:377. https://doi.org/10.3390/GEOSCIENCES9090377
Tiwari N, Satyam N (2020) An experimental study on the behavior of lime and silica fume treated coir geotextile reinforced expansive soil subgrade. Eng Sci Technol an Int J 23:1214–1222. https://doi.org/10.1016/j.jestch.2019.12.006
Tiwari N, Satyam N (2021) The coupling effect of pond-ash and polypropylene fiber on strength and durability attributes of expansive subgrades: an integrated experimental and machine learning approach. J Rock Mech Geotech Eng
Tiwari N, Satyam N, Kumar Shukla S (2020a) An experimental study on micro-structural and geotechnical characteristics of expansive clay mixed with EPS granules. Soils Found 60:705–713. https://doi.org/10.1016/j.sandf.2020.03.012
Tiwari N, Satyam N, Patva J (2020b) Engineering characteristics and performance of polypropylene fibre and silica fume treated expansive soil subgrade. Int J Geosynth Gr Eng 6:1–11. https://doi.org/10.1007/s40891-020-00199-x
Tiwari N, Satyam N, Singh K (2020c) Effect of curing on micro-physical performance of polypropylene fiber reinforced and silica fume stabilized expansive soil under freezing thawing cycles. Sci Rep 10:7624. https://doi.org/10.1038/s41598-020-64658-1
Tiwari N, Satyam N, Puppala AJ (2021a) Effect of synthetic geotextile on stabilization of expansive subgrades: an experimental study. J Mater Civ Eng. https://doi.org/10.1061/(ASCE)MT.1943-5533.0003901
Tiwari N, Satyam N, Puppala AJ (2021b) Strength and durability assessment of expansive soil stabilized with recycled ash and natural fibers. Transp Geotech 100556. https://doi.org/10.1016/j.trgeo.2021.100556
Tobler DJ, Cuthbert MO, Greswell RB et al (2011) Comparison of rates of ureolysis between Sporosarcina pasteurii and an indigenous groundwater community under conditions required to precipitate large volumes of calcite. Geochim Cosmochim Acta 75:3290–3301. https://doi.org/10.1016/j.gca.2011.03.023
Van Paassen LA (2009) Biogrout: ground improvement by microbially induced carbonate precipitation. Delft, Netherlands
Wen K, Li Y, Liu S et al (2019a) Evaluation of MICP treatment through EC and pH tests in urea hydrolysis process. Environ Geotech:1–8. https://doi.org/10.1680/jenge.17.00108
Wen K, Li Y, Liu S et al (2019b) Development of an improved immersing method to enhance microbial induced calcite precipitation treated sandy soil through multiple treatments in low cementation media concentration. Geotech Geol Eng 37:1015–1027. https://doi.org/10.1007/s10706-018-0669-6
Whiffin VS (2004) Microbial CaCO3 precipitation for the production of biocement. Ph.D. Thesis, Murdoch University
Whitman WB, Coleman DC, Wiebe WJ (1998) Prokaryotes: the unseen majority. In: Proceedings of the National Academy of Sciences of the United States of America. pp 6578–83
Wolok E, Barafi J, Joshi N et al (2020) Study of bio-materials for removal of the oil spill. Arab J Geosci 13:1244. https://doi.org/10.1007/s12517-020-06244-3
Xiao Y, Stuedlein AW, Ran J et al (2019a) Effect of particle shape on strength and stiffness of biocemented glass beads. J Geotech Geoenviron Eng 145:06019016. https://doi.org/10.1061/(ASCE)GT.1943-5606.0002165
Xiao Y, Wang Y, Desai CS et al (2019b) Strength and deformation responses of biocemented sands using a temperature-controlled method. Int J Geomech 19:04019120. https://doi.org/10.1061/(asce)gm.1943-5622.0001497
Xiao Y, Chen H, Stuedlein AW et al (2020) Restraint of particle breakage by biotreatment method. J Geotech Geoenviron Eng 146:04020123. https://doi.org/10.1061/(ASCE)GT.1943-5606.0002384
Yasuhara H, Neupane D, Hayashi K, Okamura M (2012) Experiments and predictions of physical properties of sand cemented by enzymatically-induced carbonate precipitation. Soils Found 52:539–549 https://doi.org/10.1016/j.sandf.2012.05.011
Zeebe RE (2011) On the molecular diffusion coefficients of dissolved CO2; HCO3 and CO3 and their dependence on isotopic mass. Geochim Cosmochim Acta 75:2483–2498. https://doi.org/10.1016/j.gca.2011.02.010
Zhao Q, Li L, Li C et al (2014) Factors affecting improvement of engineering properties of MICP-treated soil catalyzed by bacteria and urease. J Mater Civ Eng 26:04014094. https://doi.org/10.1061/(ASCE)MT.1943-5533
Acknowledgements
The authors would like to acknowledge the support of Dr. Saikat Sarkar, Assistant Professor, Department of Civil Engineering, IIT Indore, for providing his valuable suggestions and guidance in modeling. The authors would also acknowledge the support of Ministry of Human Resource Development, the Government of India, for funding the Ph.D. Scholarship of the first author.
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Sharma, M., Satyam, N., Tiwari, N. et al. Simplified biogeochemical numerical model to predict pore fluid chemistry and calcite precipitation during biocementation of soil. Arab J Geosci 14, 807 (2021). https://doi.org/10.1007/s12517-021-07151-x
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DOI: https://doi.org/10.1007/s12517-021-07151-x