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Initial stages of DNA Base Excision Repair in Nucleosomes

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Abstract

In mammalian cells, base excision repair (BER) is the main pathway responsible for the correction of a variety of chemically modified DNA bases. DNA packaging in chromatin affects the accessibility of damaged sites to the enzymes involved in repair processes. This review presents data concerning the enzymes involved in BER. Within the nucleosome core particle (NCP), the accessibility of damaged DNA to enzymes is hindered by the presence of a histone octamer. This means that the removal of DNA lesions largely depends on their rotational and translational positioning in the NCP, as well as on the specific features of each enzyme.

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Funding

The work was supported by the Ministry of Science and Higher Education of the Russian Federation (project no. MD-3775.2019.4) and partially supported by budgetary funding (project no. АААА-А17-117020210022-4).

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  1. Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences, 630090, Novosibirsk, Russia

    O. A. Kladova, N. A. Kuznetsov & O. S. Fedorova

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  3. O. S. Fedorova
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Correspondence to O. S. Fedorova.

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Translated by N. Onishchenko

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Kladova, O.A., Kuznetsov, N.A. & Fedorova, O.S. Initial stages of DNA Base Excision Repair in Nucleosomes. Mol Biol 55, 167–181 (2021). https://doi.org/10.1134/S0026893321020096

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  • DOI: https://doi.org/10.1134/S0026893321020096

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