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Genomic insights into the molecular mechanisms of a Pseudomonas strain significant in its survival in Kongsfjorden, an Arctic fjord

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Abstract

Whole-genome sequence of Pseudomonas sp. Kongs-67 retrieved from Kongsfjorden, an Arctic fjord, has been investigated to understand the molecular machinery required for microbial association and survival in a polar fjord. The genome size of Kongs-67 was 4.5 Mb and was found to be closely related to the Antarctic P. pelagia strain CL-AP6. This genome encodes for chemotaxis response regulator proteins (CheABB1RR2VWYZ), chemoreceptors (methyl-accepting chemotaxis proteins), and flagellar system proteins (FliCDEFGOPMN, FlhABF, FlgBCDEFGHIJKL, and MotAB proteins) vital in cellular interactions in the dynamic fjord environment. A high proportion of genes were assigned to biofilm formation (pgaABCD operon) and signal transduction protein categories (EnvZ/OmpR, CpxA/CpxR, PhoR/PhoB, PhoQ) indicating that the biofilm formation in Kongs-67 could be tightly regulated in response to the availability of signalling-metabolites. The genome of Kongs-67 encoded for HemBCD, CbiA, CobABNSTOQCDP, and BtuBFR proteins involved in cobalamin biosynthesis and transport along with proteins for siderophore-mediated iron channelling (PchR, Fur protein, FpvA); crucial in a microbial association. The genomes of Arctic strain Kongs-67 and Antarctic strain CL-AP6 were similar which is indicative of retainment of the core genes in the polar Pseudomonas strains that could be vital in conferring evolutionary adaptation for its survival in a polar fjord. Thus, our study contributes to the knowledge on the genetics of a polar Pseudomonas member exhibiting biosynthetic potentials and suggest Pseudomonas sp. Kongs-67 as a suitable candidate for the investigation of functional aspects of molecular adaptations in the polar marine environment.

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Data availability

The complete genome sequence of Pseudomonas sp. Kongs-67 has been deposited at GenBank under the accession number CP033116 (BioProject ID: PRJNA497361 and BioSample ID: SAMN10255692) and available at NCBI website (https://www.ncbi.nlm.nih.gov/genome/).

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Acknowledgements

The authors wish to express their gratitude to the Director, NCPOR, Ministry of Earth Sciences, Government of India for his interest and support for this work. The authors are grateful to Ross-Lynne Gibb née Weston, PhD candidate, Nelson Mandela University, South Africa for editing the English. This is NCPOR contribution number J-4/2021-22.

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Funding was provided by the Ministry of Earth Sciences, Govt. of India, New Delhi, India.

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  1. National Centre for Polar and Ocean Research, Ministry of Earth Sciences, Headland Sada, Vasco da Gama, 403804, Goa, India

    Rupesh Kumar Sinha & K. P. Krishnan

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  1. Rupesh Kumar Sinha
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  2. K. P. Krishnan
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Correspondence to K. P. Krishnan.

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Sinha, R.K., Krishnan, K.P. Genomic insights into the molecular mechanisms of a Pseudomonas strain significant in its survival in Kongsfjorden, an Arctic fjord. Mol Genet Genomics 296, 893–903 (2021). https://doi.org/10.1007/s00438-021-01788-9

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