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The Sigma-1 Receptor Ligand Chlorpromazine Attenuates Store-Dependent Ca2+ Entry in Peritoneal Macrophages

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Abstract

Using Fura-2AM, a fluorescent Ca2+ indicator, we have shown for the first time that sigma-1 receptor antagonist - neuroleptic chlorpromazine - significantly inhibits the store-dependent Ca2+ entry induced by immunomodulators glutoxim as well as molixan and endoplasmic reticulum Са2+-ATPase inhi-bitors thapsigargin and cyclopiazonic acid in rat peritoneal macrophages. The results suggest the involvement of sigma-1 receptors in the regulation of store-dependent Ca2+ entry in macrophages.

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Funding

This work was carried out within the framework of the research program of the Department of Biophysics of St. Petersburg State University and the Department of Clinical Biochemistry and Laboratory Diagnostics of the Military Medical Academy named after S.M. Kirov (St. Petersburg), as well as the Contract for the implementation of research works, project no. 28-12-38.

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Authors and Affiliations

  1. St. Petersburg State University, 199034, St. Petersburg, Russia

    L. S. Milenina, Z. I. Krutetskaya & N. I. Krutetskaya

  2. Kirov Military Medical Academy, 194044, St. Petersburg, Russia

    V. G. Antonov

Authors
  1. L. S. Milenina
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  2. Z. I. Krutetskaya
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  3. V. G. Antonov
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  4. N. I. Krutetskaya
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Corresponding authors

Correspondence to L. S. Milenina or Z. I. Krutetskaya.

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CONFLICT OF INTEREST

The authors declare that there is no conflict of interest.

COMPLIANCE WITH ETHICAL STANDARDS

The animals and all manipulations with them were carried out in accordance with the regulations and requirements of the Order of Ministry of Health of the Russian Federation No. 267 of 19.06.03 “On approval of rules for laboratory practice in the Russian Federation.”

Additional information

Translated by E. Puchkov

Abbreviation: [Ca2+]i, intracellular concentration of Ca2+.

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Milenina, L.S., Krutetskaya, Z.I., Antonov, V.G. et al. The Sigma-1 Receptor Ligand Chlorpromazine Attenuates Store-Dependent Ca2+ Entry in Peritoneal Macrophages. BIOPHYSICS 66, 77–83 (2021). https://doi.org/10.1134/S0006350921010115

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  • DOI: https://doi.org/10.1134/S0006350921010115

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